牙源性囊肿起源于牙源性组织，是排列有上皮细胞并被纤维结缔组织包围的病理性空洞。本研究调查了 CITED1 蛋白在不同类型的牙源性囊肿中的表达。切除了 40 个角化囊肿、40 个根状囊肿和 40 个含牙囊肿，并进行常规石蜡包埋方案处理。宏观和全景放射线图像用于诊断。记录人口统计特征和牙科参数。囊性组织用苏木精-伊红染料和 CITED1 抗体染色。对免疫染色进行半定量分析。使用Cytoscape软件进行蛋白质-蛋白质相互作用网络、hub基因检测和KEGG分析。牙源性角化囊肿成像具有6-8层上皮细胞和纤维囊肿壁与炎症细胞。根性囊肿具有不同厚度的复层鳞状上皮、纤毛细胞和Rushton透明体。含牙囊肿呈现增生性非角化上皮、纤维组织、网脊和炎性细胞。 CITED1 免疫表达在牙源性角化囊肿中最高，其次是根性囊肿，在含牙囊肿中最低。与根性囊肿和含齿囊肿相比，牙源性角化囊肿的核和细胞质 CITED1 表达显着升高。 CITED1 的前五个靶标已被确定，主要显示激素和癌症相关途径的富集。所有三种类型的牙源性囊肿中 CITED1 的阳性表达表明 CITED1 在牙源性囊肿的发病机制中具有潜在作用，特别是在角化囊肿中。需要进一步研究来阐明 CITED1 差异表达的确切机制及其对牙源性囊肿发生和进展的影响。© 2024。作者。

Originating from odontogenic tissue, Odontogenic cysts are pathological cavities lined with epithelial cells and surrounded by fibrous connective tissue. This study investigated expression of CITED1 protein in different types of odontogenic cysts.40 keratocysts, 40 radicular cysts, and 40 dentigerous cysts were excised and processed for routine paraffin wax embedding protocol. Macroscopic and panoramic radiographies images were used for diagnosis. Demographical properties and dental parameters were recorded. Cystic tissues were stained with hematoxylin-eosin dye and CITED1 antibody. Semi-quantitative analysis was performed for immune staining. The protein-protein interaction network, hub gene detection and KEGG analysis were conducted using Cytoscape software.Odontogenic keratocysts was imaged with 6-8 layered epithelial cells and fibrous cyst walls with inflammatory cells. Radicular cysts had stratified squamous epithelium with varying thickness, ciliated cells, and Rushton hyaline bodies. Dentigerous cysts presented hyperplastic non-keratinized epithelium, fibrous tissue, rete ridges, and inflammatory cells. CITED1 immunoexpression was highest in odontogenic keratocysts, followed by radicular cysts, and lowest in dentigerous cysts. Nuclear and cytoplasmic CITED1 expression was significantly elevated in odontogenic keratocysts compared to radicular and dentigerous cysts. The top five targets of CITED1 were identified, primarily showing enrichment in hormone and cancer related pathways.Positive CITED1 expression in all three types of odontogenic cysts suggest a potential role for CITED1 in the pathogenesis of odontogenic cysts, particularly in keratocysts. Further investigations are needed to elucidate the exact mechanisms underlying the differential expression of CITED1 and its implications for the development and progression of odontogenic cysts.© 2024. The Author(s).