昆虫作为替代蛋白质和生物活性化合物（主要是抗癌剂）的新来源而备受关注。富含蛋白质的昆虫幼虫血淋巴是制药和食品工业相关研究的潜在候选者。在本研究中，分析了黄粉虫和黄粉虫两种黄粉虫幼虫血淋巴的生化特性和细胞毒性。测定总蛋白质和碳水化合物、抗氧化能力和脂质过氧化水平。用不同浓度的幼虫血淋巴蛋白处理人类癌症 (U-87) 和正常代谢 (MRC-5) 细胞，并在处理后 24、48 和 72 小时测定对细胞活力的影响。家蚕血淋巴的总蛋白质含量更高，比总碳水化合物含量更高的家蚕血淋巴具有更高的抗氧化能力和脂质过氧化水平。细胞毒性测定表明，T. molitor 和 Z. morio 血淋巴以细胞类型、剂量和时间依赖性方式不同地影响 U-87 和 MRC-5 细胞的活力。两个物种的血淋巴对 U-87 细胞的细胞毒性高于对 MRC-5 细胞的细胞毒性，48 小时后尤其明显。此外，与家蚕血淋巴相比，家蚕血淋巴在两种细胞系上均观察到更有效的细胞毒性作用，这可能是由于其更高的抗氧化能力。

Insects are of great interest as novel sources of alternative proteins and biologically active compounds, primarily anticancer agents. Protein-rich insect larval hemolymph is a prospective candidate for pharmaceutical and food industry-related research. In this study, selected biochemical properties and cell toxicity of larval hemolymph from two mealworm species, Tenebrio molitor and Zophobas morio, were analyzed. Total proteins and carbohydrates, antioxidant capacity, and the level of lipid peroxidation were determined. Human cancer (U-87) and normometabolic (MRC-5) cells were treated with different concentrations of larval hemolymph proteins, and the effects on cell viability were assayed 24, 48, and 72 h after treatments. Z. morio hemolymph was shown to be richer in total proteins, showing a higher antioxidant capacity and lipid peroxidation level than T. molitor hemolymph, which was richer in total carbohydrates. Cytotoxicity assays showed that T. molitor and Z. morio hemolymphs differently affect the viability of U-87 and MRC-5 cells in cell type-, dose-, and time-dependent manners. Hemolymph from both species was more cytotoxic to U-87 cells than to MRC-5 cells, which was particularly prominent after 48 h. Additionally, a more potent cytotoxic effect of Z. morio hemolymph was observed on both cell lines, likely due to its higher antioxidant capacity, compared to T. molitor hemolymph.