结直肠癌 (CRC) 是全球第二大死亡率和第三大常见癌症，结直肠癌是一种非常异质的疾病，肿瘤可由环境和遗传风险因素形成，包括表观遗传和遗传变异。 DNA结合蛋白（ID）抑制剂是一类螺旋-环-螺旋转录调节因子；这些蛋白质被认为是四个高度保存的转录调节因子 (ID1-4) 的家族，在与肿瘤发展相关的许多过程中发挥着重要作用。 ID 家族作为其他必需 HLH 蛋白的负显性拮抗剂，导致非功能性异二聚体的产生和转录过程的调节。120 新鲜组织和血液样本 从诊断为 HLH 的患者中收集了四十 (40) 个新鲜组织和血液样本。 CRC，从诊断为健康的患者身上采集了二十 (20) 个样本。 (qRT-PCR) 方法是一种用于定量稳态 mRNA 水平的灵敏技术，用于评估 ID (1-4) 基因的表达水平。研究结果表明组织中 ID1 的下调，且在患者和对照组的血液样本中显示 ID1 基因的上调。ID2 基因也表现出高度显着的变化，其中组织中的上调和血液样本中的下调。 ID3和ID4基因在组织和血液样本中表现出下调，其中ID3血液样本在患者和血型之间存在显着变化。由于ID家族在许多过程中的调节功能，肿瘤中IDs基因的上调或下调证明了如何对于肿瘤的发展非常重要，因此这些蛋白质可以用作 CRC 的指示剂。© 2024。作者获得 Springer Nature B.V. 的独家许可。

Colorectal cancer (CRC) is the second most deathly worldwide and third most common cancer, CRC is a very heterogeneous disease where tumors can form by both environmental and genetic risk factors and includes epigenetic and genetic alternations. Inhibitors of DNA binding proteins (ID) are a class of helix-loop-helix transcription regulatory factors; these proteins are considered a family of four highly preserved transcriptional regulators (ID1-4), shown to play significant roles in many processes that are associated with tumor development. ID family plays as negatively dominant antagonists of other essential HLH proteins, concluding the creation of non-functional heterodimers and regulation of the transcription process.120 Fresh tissue and blood samples Forty (40) samples of fresh tissue and blood were collected from patients diagnosed with CRC, twenty (20) samples were collected from a patient diagnosed as healthy. The (qRT-PCR) method is a sensitive technique for the quantifying of steady-state mRNA levels that used to evaluation the expression levels of ID (1-4) gene.The findings indicate downregulation in ID1 in tissue with a highly significant change between patients and control groups, where upregulation in the ID1 gene is shown in blood samples.ID2 gene also demonstrated high significant change where show upregulation in tissue and downregulation in blood sample. ID3 and ID4 genes show downregulation in tissue and blood samples with a significant change in ID3 blood samples between patient and blood groups.Because of the regulation function of the ID family in many processes, the up or down regulation of IDs genes in tumors Proves how important its tumor development, and therefore those proteins can be used as an indicator for CRC.© 2024. The Author(s), under exclusive licence to Springer Nature B.V.