LncRNA在多种疾病中具有有效价值，早已应用于前列腺癌的诊断、治疗和预后。本研究重点关注lncRNA PITPNA-AS1，探讨其在前列腺癌中的诊断潜力。通过实时定量聚合酶链反应（PCR）测定前列腺癌血清和样本细胞中PITPNA-AS1和miR-129-5p的表达（ RT-qPCR）。考虑PITPNA-AS1的表达与临床病理参数之间的关系。 ROC曲线提示PITPNA-AS1的诊断价值。使用体外细胞测定验证了 PITPNA-AS1 对前列腺癌细胞的作用。荧光素酶活性测定和RIP测定表明PITPNA-AS1与miR-129-5p之间存在海绵关系。前列腺癌中PITPNA-AS1水平升高，而miR-129-5p水平明显降低。 PITPNA-AS1表达与患者的Gleason分级、淋巴结转移和TNM分期相关。曲线下面积（AUC）为0.910，具有较高的敏感性和特异性。 PITPNA-AS1 被阐明直接靶向 miR-129-5p，而沉默 PITPNA-AS1 会对前列腺癌细胞的增殖、迁移和侵袭产生负面影响。 miR-129-5p抑制剂的干预逆转了PITPNA-AS1沉默对细胞的影响。PITPNA-AS1在前列腺癌中相对高表达，介导患者的病理生理过程，可作为诊断指标。 PITPNA-AS1 海绵 miR-129-5p 的沉默会抑制细胞的生物学功能，表明 PITPNA-AS1 可能代表前列腺癌的新治疗靶点。© 2024。作者。

LncRNA has an effective value in many diseases, which has long been applied in the diagnosis, treatment and prognosis of prostate cancer. This study focused on lncRNA PITPNA-AS1, and its diagnostic potential in prostate cancer has been explored.The expression of PITPNA-AS1 and miR-129-5p in prostate cancer serum and sample cells was determined by real-time quantitative polymerase chain reaction (RT-qPCR). The relationship between the expression of PITPNA-AS1 and clinicopathological parameters was considered. ROC curve prompted the diagnostic value of PITPNA-AS1. The effect of PITPNA-AS1 on prostate cancer cells was verified using vitro cells assay. Luciferase activity assay and RIP assay demonstrated the sponge relationship of PITPNA-AS1 to miR-129-5p.PITPNA-AS1 level was increased, while miR-129-5p was obviously decreased in prostate cancer. PITPNA-AS1 expression was associated with Gleason grade, lymph node metastasis and TNM stage in patients. The area under the curve (AUC) was 0.910, with high sensitivity and specificity. PITPNA-AS1 was elucidated to directly target miR-129-5p, whereas silencing PITPNA-AS1 negatively affected prostate cancer cell proliferation, migration and invasion. Intervention of miR-129-5p inhibitor reversed the effect of silencing PITPNA-AS1 on cells.PITPNA-AS1 was relatively highly expressed in prostate cancer and mediated the pathophysiological process of patients, which may serve as a diagnostic indicator. Silencing of the PITPNA-AS1 sponge miR-129-5p inhibited the biological function of the cells, indicating that PITPNA-AS1 may represent a novel therapeutic target for prostate cancer.© 2024. The Author(s).