在这项研究中，开发了一种新的三重发夹寡聚传感器，用于测定乳腺癌生物标志物，使用硅量子点 (Si QD)（λex = 370 nm，λem = 482 nm）作为供体，金纳米颗粒 (GNP) 作为受体。 FRET（荧光共振能量转移）机制。在三链体发夹寡核苷酸传感器中，用 Si QD 标记的三链体形成寡核苷酸 (TFO) 和用 GNP 标记的单链 DNA 形成发夹形状，在发夹茎处具有三链体结构。在开启机制中，三重发夹茎在序列特异性 miRNA-155 存在的情况下打开，导致 Si QD 标记的 TFO 探针的释放和荧光信号的恢复。 Si QD-TFO 约 80% 的荧光强度在寡传感器的三重发夹结构中被猝灭，并且在 800 pM miRNA-155 存在的情况下，荧光信号恢复至其初始值的 57.7%。获得约 10 pM 的 LOD。设计的基于三重的生物传感器可以高选择性地区分乳腺癌生物标志物的浓度。版权所有 © 2024 Elsevier B.V. 保留所有权利。

In this study, a new triplex hairpin oligosensor was developed for the determination of a breast cancer biomarker using silicon quantum dots (Si QD) (λex = 370 nm, λem = 482 nm) as donor and gold nanoparticles (GNP) as an acceptor in a FRET (fluorescence resonance energy transfer) mechanism. In the triplex hairpin oligosensor, a triplex-forming oligonucleotide (TFO) labeled with Si QD and a single-strand DNA labeled with GNP form a hairpin shape with a triplex structure at the hairpin stem. In a turn-on mechanism, the triplex hairpin stem is opened in the presence of sequence-specific miRNA-155 which leads to the release of the Si QD-labeled TFO probe and recovery of the fluorescence signal. About 80 % of the fluorescence intensity of the Si QD-TFO is quenched in the triplex hairpin structure of the oligosensor and in the presence of 800 pM miRNA-155, the fluorescence signal recovered to 57.7 % of its initial value. The LOD of about 10 pM was obtained. The designed triplex-based biosensor can discriminate concentrations of breast cancer biomarkers with high selectivity.Copyright © 2024 Elsevier B.V. All rights reserved.