植物化学分析，体外和硅内研究对乙酸乙酸乙酯乙酸乙酸乙酯的抗增殖活性（Hochst。Exoliv。和Hiern）C.Jeffrey针对人宫颈癌细胞系

简介：由于当前治疗方法及其相关的不良副作用，宫颈癌是全球妇女死亡的主要原因之一。洗衣店用作传统医学，以治疗包括癌症在内的各种疾病。然而，尚无科学验证对颈癌对宫颈癌的抗增殖活性。目的：这项研究旨在通过网络分析方法评估L. cornuta的选择性抗增生性，细胞毒性和抗移民作用，并通过网络分析方法探索其在人宫颈癌细胞系（HELA-229）中的治疗机制。材料和方法：通过3-（4、5-二甲基硫代基-2-基）-2，5-二甲基甲基甲唑烷溴化物（MTT）生物鉴定和抗术效果评估抗术效果的细胞毒性对宫颈癌细胞增殖的细胞毒性影响。使用定性颜色方法和气相色谱 - 质谱法（GC-MS）分析化合物。随后，对基因和基因组的京都百科全书（KEGG）分析（包括蛋白质 - 蛋白质相互作用（PPI）网络分析，基因本体学（GO）（GO）（GO）分析（包括蛋白质 - 蛋白质相互作用（PPI）相互作用（PPI）分析）进行了筛查，以进行筛查，以进行筛查，以进行潜在的抗癌癌症治疗靶基因。进行分子对接（MD）以预测和了解配体针对宫颈癌的分子相互作用。进行逆转录 - 定量聚合酶链反应（RT-QPCR）以验证网络分析结果。结果：乙酸甲酯乙酸酯馏分对HELA-229增殖（IC50的20.56±2.83μg/ml）表现出显着的细胞毒性作用，其选择性指数（SI）为2.36，为2.36，对非ccastorialion-climion Simart simimal细胞毒性（VERO-CCL 81（VERO-CCL 81）（IC50）（IC50）（IC50）（IC502）（IC502）（IC）（IC502）。通过GC-MS分析，还鉴定出糖苷，苯酚，皂苷，萜类化合物，喹酮和单宁。 PI3K/AKT1，RAS/MAPK，FOXO和EGFR信号通路被确定为显着富集的途径。 affinity against TP53 and P21 with -7.0 and -8.0 kcal/mol, respectively. This suggests a stable molecular interaction of the ethyl acetate fraction of L. cornuta compounds with the selected target genes for cervical cancer. Furthermore, RT-qPCR analysis revealed that CDK2, MDM2 and BCL2 were downregulated, and Casp9 and P21 were upregulated in与阴性对照相比，用L. cornuta处理的HELA-229细胞（DMSO 0.2％）。

Introduction: Cervical cancer is one of the leading causes of death among women globally due to the limitation of current treatment methods and their associated adverse side effects. Launaea cornuta is used as traditional medicine for the treatment of a variety of diseases including cancer. However, there is no scientific validation on the antiproliferative activity of L. cornuta against cervical cancer. Objective: This study aimed to evaluate the selective antiproliferative, cytotoxic and antimigratory effects of L. cornuta and to explore its therapeutical mechanisms in human cervical cancer cell lines (HeLa-229) through a network analysis approach. Materials and methods: The cytotoxic effect of L. cornuta ethyl acetate fraction on the proliferation of cervical cancer cells was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) bioassay and the antimigratory effect was assessed by wound healing assays. Compounds were analysed using the qualitative colour method and gas chromatography-mass spectroscopy (GC-MS). Subsequently, bioinformatic analyses, including the protein-protein interaction (PPI) network analysis, Gene Ontology (GO), and Kyoto Encyclopaedia of Genes and Genomes (KEGG) analysis, were performed to screen for potential anticervical cancer therapeutic target genes of L. cornuta. Molecular docking (MD) was performed to predict and understand the molecular interactions of the ligands against cervical cancer. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to validate the network analysis results. Results: L. cornuta ethyl acetate fraction exhibited a remarkable cytotoxic effect on HeLa-229 proliferation (IC50 of 20.56 ± 2.83 μg/mL) with a selectivity index (SI) of 2.36 with minimal cytotoxicity on non-cancerous cells (Vero-CCL 81 (IC50 of 48.83 ± 23.02). The preliminary screening revealed the presence of glycosides, phenols, saponins, terpenoids, quinones, and tannins. Thirteen compounds were also identified by GC-MS analysis. 124 potential target genes associated with the effect of L. cornuta ethyl acetate fraction on human cervical cancer were obtained, including AKT1, MDM2, CDK2, MCL1 and MTOR were identified among the top hub genes and PI3K/Akt1, Ras/MAPK, FoxO and EGFR signalling pathways were identified as the significantly enriched pathways. Molecular docking results showed that stigmasteryl methyl ether had a good binding affinity against CDK2, ATK1, BCL2, MDM2, and Casp9, with binding energy ranging from -7.0 to -12.6 kcal/mol. Tremulone showed a good binding affinity against TP53 and P21 with -7.0 and -8.0 kcal/mol, respectively. This suggests a stable molecular interaction of the ethyl acetate fraction of L. cornuta compounds with the selected target genes for cervical cancer. Furthermore, RT-qPCR analysis revealed that CDK2, MDM2 and BCL2 were downregulated, and Casp9 and P21 were upregulated in HeLa-229 cells treated with L. cornuta compared to the negative control (DMSO 0.2%). Conclusion: The findings indicate that L. cornuta ethyl acetate fraction phytochemicals modulates various molecular targets and pathways to exhibit selective antiproliferative and cytotoxic effects against HeLa-229 cells. This study lays a foundation for further research to develop innovative clinical anticervical cancer agents.