用化疗和手术治疗恶性肿瘤通常与疾病复发和转移有关。免疫疗法通过对肿瘤抗原产生积极反应来改善癌症治疗。各种癌细胞表面表达大量的葡萄糖调节蛋白78（GRP78）蛋白。刺激免疫系统对抗这种抗原可以使癌细胞暴露于免疫系统。在此，我们研究了基于 cGRP78 的疫苗针对不同癌细胞的有效性。用 cGRP78 对 BALB/c 小鼠进行免疫。通过细胞ELISA评估针对不同癌细胞的体液免疫反应。通过用不同的癌症抗原进行脾细胞增殖测定来确定细胞免疫反应。通过将黑色素瘤癌细胞注射到小鼠尾部，在接种疫苗的小鼠中研究疫苗接种对转移的影响。这些结果表明cGRP78具有可接受的抗原性并刺激免疫系统产生抗体。注射3次后，产生的抗体量与对照组有显着差异。与其他三种细胞类型相比，Hela 和 HepG2 对接种疫苗的小鼠血清表现出最高的反应。与其他细胞相比，针对 B16F10 细胞系的细胞免疫效果最好。转移结果显示，30天后，接种小鼠的肺组织中B16F10黑色素瘤癌细胞的生长并不明显。考虑到接种小鼠对转移的抵抗力，该疫苗通过抑制癌细胞的扩散，为癌症治疗提供了广阔的前景。癌细胞。版权所有：© 2024 Research in Pharmaceutical Sciences。

Treatment of malignancies with chemotherapy and surgery is often associated with disease recurrence and metastasis. Immunotherapy improves cancer treatment by creating an active response against tumor antigens. Various cancer cells express a large amount of glucose-regulated protein 78 (GRP78) protein on their surface. Stimulating the immune system against this antigen can expose cancer cells to the immune system. Herein, we investigated the effectiveness of a cGRP78-based vaccine against different cancer cells.BALB/c mice were immunized with the cGRP78. The humoral immune response against different cancer cells was assessed by Cell-ELISA. The cellular immunity response was determined by splenocyte proliferation assay with different cancer antigens. The effect of vaccination on metastasis was investigated in vaccinated mice by injecting melanoma cancer cells into the tail of mice.These results indicated that the cGRP78 has acceptable antigenicity and stimulates the immune system to produce antibodies. After three injections, the amount of produced antibody was significantly different from the control group. Compared to the other three cell types, Hela and HepG2 showed the highest reaction to the serum of vaccinated mice. Cellular immunity against the B16F10 cell line had the best results compared to other cells. The metastasis results showed that after 30 days, the growth of B16F10 melanoma cancer cells was not noticeable in the lung tissue of vaccinated mice.Considering the resistance of vaccinated mice to metastasis, this vaccine offers a promising prospect for cancer treatment by inhibiting the spread of cancer cells.Copyright: © 2024 Research in Pharmaceutical Sciences.