Hyphaene thebaica还原纳米钐钡的潜在纳米医学应用及其物理化学特性
Potential nanomedicinal applications and physicochemical nature of Hyphaene thebaica-reduced nano-samaria
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影响因子:2.1
分区:工程技术3区 / 显微镜技术2区 解剖学与形态学3区 生物学4区
发表日期:2024 Dec
作者:
Hamza Elsayed Ahmad Mohamed, Ali Talha Khalil, Khaoula Hkiri, Muhammad Ayaz, Assad Usman, Abdul Sadiq, Farhat Ullah, Muhammad Arif Khan, Ikram Ullah, Malik Maaza
DOI:
10.1002/jemt.24654
摘要
本研究采用生态友好且绿色的方法生物合成了钐氧化物纳米粒子(HT-Sm2O3 NPs),以Hyphaene thebaica果实水提取物作为螯合剂。对所制备的纳米粒子进行了多种物理化学性质分析,并评估其在生物医学中的潜在应用。X射线衍射(XRD)谱显示出与粉末衍射标准(JCPDS)卡片编号00-042-1464一致的锐峰。Debye-Scherrer和Williamson-Hall(W-H)法估算的晶粒尺寸分别为28.73nm和69.3nm。采用Kubelka-Munk(K-M)函数计算得带隙约为4.58 eV。拉曼光谱在121、351、424和561 cm-1处观察到振动位移。光致发光(PL)谱在360和540 nm处有两个主要峰值。高分辨透射电子显微镜(HR-TEM)分析显示,HT-Sm2O3纳米粒子主要呈球形到立方体形状。选区电子衍射(SAED)图案显示点状环,表明这些纳米粒子具有高度结晶性。通过多种生物测定测试其潜在的纳米药物应用,包括抗氧化活性(在1000μg/mL下抑制率为45.71%±1.13%)、盐水虾毒性(最高细胞毒性46.67%±3.33%在1000μg/mL,LC50为1081μg/mL)、血管生成抑制(20.41%±1.18%在1000μg/mL)、MTT细胞毒性(对3T3细胞IC50为104.6μg/mL,对MFC-7细胞IC50为413.25μg/mL)、乙酰胆碱酯酶(AChE)抑制(IC50为320μg/mL)、以及抗糖尿病作用(α-葡萄糖苷酶和α-淀粉酶抑制,IC50分别为380μg/mL和952μg/mL)。总体而言,本研究表明,Sm2O3纳米粒子具有中等抗癌、胆碱酯酶抑制和抗糖尿病潜能,仍需进一步评估。研究亮点:本工作采用生态友好的方法合成纳米钐,利用多种技术(如拉曼、HR-TEM、FTIR、DRS、XRD等)对其性质进行表征,并通过多种体外生物测定评估其在糖尿病、阿尔茨海默和癌症中的潜在应用,显示出良好的潜在生物医学应用潜力。
Abstract
Herein we described the biofabrication of samarium oxide nanoparticles (HT-Sm2O3 NPs) by applying the aqueous fruit extract of Hyphaene thebaica was utilized as an eco-friendly chelating agent. The prepared NPs were subjected to various physicochemical properties and potential in biomedical applications. X-ray Diffraction (XRD) pattern revealed sharp peaks that corroborated with the Joint Committee on Powder Diffraction Standards (JCPDS) card no. 00-042-1464. Crystallite size obtained from Debye-Scherrer approximation and Williamson-Hall (W-H) plot was 28.73 and 69.3 nm, respectively. Optical bandgap was calculated by employing Kubelka-Munk (K-M) function and was found to be ~4.58 eV. Raman shift was observed at 121, 351, 424-, and 561 cm-1. Photoluminescence (PL) spectra revealed two major peaks positioned at 360 and 540 nm. The high-resolution transmission electron microscopy (HR-TEM) analysis of HT-Sm2O3 nanoparticles (NPs) showed that they predominantly have spherical to cuboidal shapes. Additionally, the selected area electron diffraction (SAED) pattern presented spotty rings, indicating a high level of crystallinity in these NPs. The potential nanomedicine applications were studied using diverse bioassays using different treatments. The antioxidant activity demonstrated 45.71% ± 1.13% inhibition at 1000 μg/mL. Brine shrimp lethality assay revealed the highest cytotoxicity of 46.67% ± 3.33% at 1000 μg/mL and LC50 value of 1081 μg/mL. HT-Sm2O3 NPs exhibited inhibition of angiogenesis (20.41% ± 1.18%) at of 1000 μg/mL. MTT assay results indicated that HT-Sm2O3 NPs exhibit inhibitory effects on cell lines. Specifically, these NPs showed an IC50 value of 104.6 μg/mL against 3T3 cells. Against MCF-7 cells, the NPs demonstrated an IC50 value of 413.25 μg/mL. Additionally, in the inhibition of acetylcholinesterase (AChE), the newly synthesized NPs showed an IC50 value of 320 μg/mL. The antidiabetic assessment through α-glucosidase and α-amylase inhibition assays revealed, an IC50 value of 380 μg/mL for α-glucosidase and 952 μg/mL for α-amylase was calculated. Overall, our study suggested that the Sm2O3 NPs possess moderate anticancer, cholinesterase inhibition, and antidiabetic potential, however, needs further assessment. RESEARCH HIGHLIGHTS: In this work, nano-samaria is synthesized using an eco-friendly and green approach. The nanoparticles were characterized using techniques such as Raman, HR-TEM, FTIR, DRS, XRD, and so on, and the applications were studied using multiple in vitro bioassays for Diabetes, Alzheimer, and Cancer. The nano-samaria revealed good potential for potential biomedical applications.