用于可视化的肝细胞癌治疗的GPC3的超声响应纳米传递系统
Ultrasound-Responsive Nanodelivery System of GPC3-Targeting and Sonosensitizer for Visualized Hepatocellular Carcinoma Therapy
影响因子:6.50000
分区:医学2区 / 药学2区 纳米科技3区
发表日期:2024
作者:
Juying Zhang, Xia Luo, Xin Yang, Hanmei Li, Qiong Jiang, You Yang, Menglin Luo, Zijun Ma, Ping He, Linli Feng, Ling Li, Maochun Zhang, Yang Li, Jinhong Yu
摘要
肝细胞癌（HCC）的发生率不断增加，死亡率仍然很高。因此，需要更有效的策略来改善HCC的治疗。在这项研究中，我们报告了可视化的Glypican-3（GPC3） - 靶向纳米递送系统（命名为GC-NBS），并结合使用声动力疗法（SDT）来增强治疗HCC的治疗效率。获得的纳米传递系统可以主动靶向肝细胞癌细胞，并通过在低强度超声辐射下向纳米泡中的相变为纳米泡实现超声成像。 Meanwhile, the released chlorine e6 (Ce6) after the nanobubbles collapse could lead to the generation of reactive oxygen species (ROS) under ultrasound irradiation to induce SDT.Both in vitro and in vivo experiments have shown that GC-NBs can accumulate in tumour areas and achieve sonodynamic antitumour therapy under the navigation action of glypican-3-antibody (GPC3-Ab).此外，体外和体内实验没有显示纳米递送系统的显着生物学毒性。此外，GC-NB可以通过超声进行成像，提供个性化的治疗监控。GC-NBS通过将肿瘤特异性的靶向和刺激反应控制的释放组合到单个系统中，从目标的超声动力学角度实现了可视化的抗肿瘤策略。
Abstract
The incidence of hepatocellular carcinoma (HCC) is continuously increasing, and the mortality rate remains high. Thus, more effective strategies are needed to improve the treatment of HCC.In this study, we report the use of a visualized glypican-3 (GPC3)-targeting nanodelivery system (named GC-NBs) in combination with sonodynamic therapy (SDT) to enhance the therapeutic efficacy for treating HCC. The obtained nanodelivery system could actively target hepatocellular carcinoma cells and achieve ultrasound imaging through phase changes into nanobubbles under low-intensity ultrasound irradiation. Meanwhile, the released chlorine e6 (Ce6) after the nanobubbles collapse could lead to the generation of reactive oxygen species (ROS) under ultrasound irradiation to induce SDT.Both in vitro and in vivo experiments have shown that GC-NBs can accumulate in tumour areas and achieve sonodynamic antitumour therapy under the navigation action of glypican-3-antibody (GPC3-Ab). Furthermore, in vitro and in vivo experiments did not show significant biological toxicity of the nanodelivery system. Moreover, GC-NBs can be imaged with ultrasound, providing personalized treatment monitoring.GC-NBs enable a visualized antitumour strategy from a targeted sonodynamic perspective by combining tumour-specific targeting and stimuli-responsive controlled release into a single system.