肝细胞癌（HCC）的发病率持续上升，死亡率居高不下。因此，需要更有效的策略来改善 HCC 的治疗。在这项研究中，我们报告了使用可视化磷脂酰肌醇蛋白聚糖 3 (GPC3) 靶向纳米递送系统（称为 GC-NB）与声动力疗法 (SDT) 相结合，以增强治疗HCC的疗效。所获得的纳米递送系统可以主动靶向肝细胞癌细胞，并在低强度超声照射下通过相变形成纳米气泡实现超声成像。同时，纳米气泡破裂后释放出的氯e6（Ce6）在超声照射下会导致活性氧（ROS）的产生，从而诱导SDT。体外和体内实验均表明GC-NBs可以在肿瘤区域积聚在磷脂酰肌醇蛋白聚糖3抗体（GPC3-Ab）的导航作用下实现声动力抗肿瘤治疗。此外，体外和体内实验并未显示纳米递送系统具有显着的生物毒性。此外，GC-NB 可以通过超声成像，提供个性化治疗监测。GC-NB 通过将肿瘤特异性靶向和刺激响应控制释放结合到单个系统中，从有针对性的声动力学角度实现可视化抗肿瘤策略。© 2024等人。

The incidence of hepatocellular carcinoma (HCC) is continuously increasing, and the mortality rate remains high. Thus, more effective strategies are needed to improve the treatment of HCC.In this study, we report the use of a visualized glypican-3 (GPC3)-targeting nanodelivery system (named GC-NBs) in combination with sonodynamic therapy (SDT) to enhance the therapeutic efficacy for treating HCC. The obtained nanodelivery system could actively target hepatocellular carcinoma cells and achieve ultrasound imaging through phase changes into nanobubbles under low-intensity ultrasound irradiation. Meanwhile, the released chlorine e6 (Ce6) after the nanobubbles collapse could lead to the generation of reactive oxygen species (ROS) under ultrasound irradiation to induce SDT.Both in vitro and in vivo experiments have shown that GC-NBs can accumulate in tumour areas and achieve sonodynamic antitumour therapy under the navigation action of glypican-3-antibody (GPC3-Ab). Furthermore, in vitro and in vivo experiments did not show significant biological toxicity of the nanodelivery system. Moreover, GC-NBs can be imaged with ultrasound, providing personalized treatment monitoring.GC-NBs enable a visualized antitumour strategy from a targeted sonodynamic perspective by combining tumour-specific targeting and stimuli-responsive controlled release into a single system.© 2024 Zhang et al.