光生物调节（PBM）已广泛用于有效治疗与癌症治疗相关的并发症，包括口腔粘膜炎、放射性皮炎和手术伤口。然而，由于 PBM 对癌细胞的影响及其机制尚不清楚，因此 PBM 抗癌的安全性仍需验证。目前的研究通过检查 B16F10 黑色素瘤细胞上的四种不同激光波长（405、532、635 和 808 nm）来研究波长依赖性 PBM 效应。体外测试表明，808 nm 的 PBM 促进 B16F10 细胞的增殖和迁移。体内结果表明，808 nm 的 PBM 显着增加了相对肿瘤体积，并通过 VEGF 和 HIF-1α 的过度表达促进血管生成。此外，PBM还诱导与癌细胞增殖和肿瘤生长密切相关的因子磷酸化，并上调相关基因的表达。目前的结果表明，与其他波长相比，808 nm 对恶性黑色素瘤具有显着的肿瘤刺激作用。进一步的研究将深入探讨PBM对肿瘤刺激的分子机制，以保证PBM用于临床癌症治疗的安全性。版权所有©2024 Elsevier B.V.保留所有权利。

Photobiomodulation (PBM) has widely been used to effectively treat complications associated with cancer treatment, including oral mucositis, radiation dermatitis, and surgical wounds. However, the safety of PBM against cancer still needs to be validated as the effects of PBM on cancer cells and their mechanisms are unclear. The current study investigated the wavelength-dependent PBM effects by examining four different laser wavelengths (405, 532, 635, and 808 nm) on B16F10 melanoma tumor cells. In vitro tests showed that PBM with 808 nm promoted both proliferation and migration of B16F10 cells. In vivo results demonstrated that PBM with 808 nm significantly increased the relative tumor volume and promoted angiogenesis with overexpression of VEGF and HIF-1α. In addition, PBM induced the phosphorylation of factors closely related to cancer cell proliferation and tumor growth and upregulated the related gene expression. The current result showed that compared to the other wavelengths, 808 nm yielded a significant tumor-stimulating effect the malignant melanoma cancer. Further studies will investigate the in-depth molecular mechanism of PBM on tumor stimulation in order to warrant the safety of PBM for clinical cancer treatment.Copyright © 2024 Elsevier B.V. All rights reserved.