细胞环境中的生物分子通过液-液相分离（LLPS）聚集自发形成液滴状结构，在多种生物过程中发挥着关键作用。这些结构与一系列疾病密切相关，包括神经退行性疾病、癌症和传染病，凸显了了解 LLPS 机制对于阐明疾病发病机制和探索潜在治疗干预措施的重要性。在这篇综述中，我们概述了 LLPS 研究的最新进展，强调其病理相关性、治疗考虑以及生物信息学工具和数据库在促进 LLPS 研究中的关键作用。此外，我们对专门用于 LLPS 研究的生物信息资源进行了全面分析，以阐明其功能和适用性。通过提供对当前 LLPS 相关生物信息学资源的全面见解，本综述强调了其对人类健康和疾病的影响。版权所有 © 2024 The Chinese Medical Association，由 Wolters Kluwer, Inc. 根据 CC-BY-NC-ND 许可制作。

Biomolecular aggregation within cellular environments via liquid-liquid phase separation (LLPS) spontaneously forms droplet-like structures, which play pivotal roles in diverse biological processes. These structures are closely associated with a range of diseases, including neurodegenerative disorders, cancer and infectious diseases, highlighting the significance of understanding LLPS mechanisms for elucidating disease pathogenesis, and exploring potential therapeutic interventions. In this review, we delineate recent advancements in LLPS research, emphasizing its pathological relevance, therapeutic considerations, and the pivotal role of bioinformatic tools and databases in facilitating LLPS investigations. Additionally, we undertook a comprehensive analysis of bioinformatic resources dedicated to LLPS research in order to elucidate their functionality and applicability. By providing comprehensive insights into current LLPS-related bioinformatics resources, this review highlights its implications for human health and disease.Copyright © 2024 The Chinese Medical Association, produced by Wolters Kluwer, Inc. under the CC-BY-NC-ND license.