本研究旨在探讨口腔鳞状细胞癌（OSCC）中三级淋巴结构（TLS）的特点及其与临床和病理特征的关系。通过分析TCGA数据库中12个TLS相关趋化因子，探讨口腔鳞状细胞癌（OSCC）中的TLS。对 189 名 OSCC 患者（其中 114 名有临床和预后信息）的大队列中 TLS 的密度、成熟度和位置进行了评估。并分析了TLSs与临床病理特征之间的意义。生物信息学和分析表明TLSs与OSCC更好的临床结果相关。组织学染色和分析显示，高密度组的总生存率（71/112，63.4％）显着高于低密度组（41/112，36.6％）（p< 0.0001），并且高密度组淋巴结转移较少（50.0%/68.3%，p = 0.021）。 TLS 根据成熟度和位置分为 4 种类型。不同类型的TLS与OSCC患者的预后（OS，p< 0.0001）、临床特征（T分期，p = 0.028；分化程度，p = 0.043）和癌前病变类型（OSF，p = 0.049）相关。 TLS 与更好的 OSCC 预后密切相关，更系统的分类可以更好地指导进一步治疗方案的制定。© 2024 John Wiley

This study aimed to investigate the characteristics of tertiary lymphoid structures (TLSs) in oral squamous cell carcinoma (OSCC) and their association with clinical and pathological features.12 TLS-related chemokines in TCGA database were analyzed to investigate the TLSs in OSCC. The density, maturity, and location of TLSs in a large cohort of 189 OSCC patients (114 of which had clinical and prognostic information) were assessed. And the significance between TLSs and clinicopathologic characteristics was analyzed.Bioinformatics and analysis showed that TLSs were associated with better clinical outcomes in OSCC. Histological staining and analysis showed that the overall survival rate of the high-density group (71/112, 63.4%) was significantly higher (p < 0.0001) than that of the low-density group (41/112, 36.6%), and the high-density group had fewer lymph node metastases (50.0%/68.3%, p = 0.021). And TLSs were divided into 4 types according to the maturity and location. Different types of TLSs are associated with prognosis (OS, p < 0.0001), clinical features (T stage, p = 0.028; degree of differentiation, p = 0.043), and precancerous lesion types (OSF, p = 0.049) of OSCC patients.TLSs were closely associated with better OSCC prognosis, and a more systematic classification may better guide the formulation of further treatment options.© 2024 John Wiley & Sons Ltd.