通过在共聚刷中利用光响应螺吡喃衍生物和 PDEGMA 的组合，细胞选择性热响应聚（二（乙二醇）甲醚甲基丙烯酸酯）（PDEGMA）细胞收获系统的性能得到显着增强。共聚1'-(2-甲基丙烯酰氧基乙基)-3',3'-二甲基-6-硝基螺(2H-1-苯并吡喃-2,2'-二氢吲哚)(SPMA) (DEMGA)二(乙二醇)甲基的分析醚甲基丙烯酸酯刷表明，当温度从 37°C 切换到 22°C 并交替使用不同的光波长（从 530 到 365 nm）照射时，SPMA/DEGMA 比例的微小调整会导致润湿性和蛋白质吸附发生显着变化。 ）。薄的 P(SPMA-co-DEGMA) 层支持胰腺肿瘤 PaTu 8988t 细胞具有高细胞活力。含有 2.5% SPMA/DEGMA 的共聚物层可实现最高效率的无酶细胞释放，并具有非常好的细胞活力。通过将细胞培养基冷却至 22°C 并用 365 nm 光照射表面来诱导释放。与纯 PDEGMA 相比，P(SPMA-co-DEGMA) 层的贴壁细胞形态变化速度增加了三倍，分离细胞比例增加了 5 倍以上，这凸显了这些双重细胞的潜力响应式 PDEGMA 系统，可在轻松捕获、培养和释放不同细胞系方面优化性能。© 2024 作者。高分子生物科学由 Wiley‐VCH GmbH 出版。

The performance of the cell-selective thermoresponsive poly(di(ethylene glycol)methyl ether methacrylate) (PDEGMA) cell harvest system is shown to be drastically enhanced by exploiting the combination of photoresponsive spiropyran derivates and PDEGMA in copolymerized brushes. The analysis of copolymerized 1'-(2-methacryloxyethyl)-3',3'-dimethyl-6-nitrospiro(2H-1-benzopyran-2,2'-indoline) (SPMA) (DEMGA) di(ethylene glycol)methyl ether methacrylate brushes revealed that a minor adjustment of the SPMA/DEGMA ratios results in a significant alternation of wettability as well as protein adsorption, when switching the temperature from 37 to 22 °C and alternately irradiating using different light wavelengths (from 530 to 365 nm). Thin P(SPMA-co-DEGMA) layers supported pancreatic tumor PaTu 8988t cells with high cell viability. Copolymer layers with 2.5% SPMA/DEGMA led to the highest efficiency of enzyme-free cell release with very good cell viability. The release is induced by cooling the cell culture medium to 22 °C and irradiating the surface with 365 nm light. Compared to neat PDEGMA, the P(SPMA-co-DEGMA) layers showed a threefold increase in the speed of the change of cell morphology of the attached cells and a >5 times increased fraction of detached cells, which underlines the potential of these dual responsive PDEGMA systems for optimized performance in the facile capture, culture, and release of different cell lines.© 2024 The Author(s). Macromolecular Bioscience published by Wiley‐VCH GmbH.