血小板激活因子（PAF）与从排卵到胚胎运动等一系列生殖过程有关，但尚未在母马中得到广泛研究。为了鉴定 PAF 的存在并检查 PAF 在马围孕过程中的作用，对马卵泡液 (FF) 进行靶向质谱联用色谱分离 (LC-MS/MS)，并定量检测 PAF。 随后，进行了基于非靶向高分辨率质谱的脂质组学分析，以量化不同大小的排卵前卵泡中的 PAF，其中发现了不同分子种类的 PAF、PAF(14:0) 和 PAF(16:1)。随卵泡直径增大。这些发现表明，随着排卵前卵泡接近排卵，FF 内的 PAF 会增加。此外，免疫荧光染色鉴定了马输卵管上皮细胞的管腔细胞周、顶端和基底方面的 PAF 受体 (PAFR)。最后，生成了马输卵管上皮类器官模型，结果表明添加 PAF 显着增加了纤毛跳动频率 (CBF) (Hz)，这一作用与 PAF 在胚胎迁移中的作用一致。据推测，PAF 对输卵管纤毛细胞的局部作用将卵母细胞和胚胎推向子宫。在母马中，PAF 似乎是围孕期的一个贡献者，可能是排卵机制和早期妊娠对话的中介者。

Platelet-activating factor (PAF) has been implicated in a number of reproductive processes ranging from ovulation to embryo motility, but has not been widely explored in the mare. To identify the presence and examine the role of PAF in the equine periconception processes, targeted mass spectrometry coupled with chromatographic separation (LC-MS/MS) was performed on equine follicular fluid (FF), and PAF was quantitatively detected. Subsequently, untargeted high-resolution mass spectrometry-based lipidomic analysis was carried out to quantify PAF in different sized pre-ovulatory follicles, whereby different molecular species of PAF, PAF(14:0) and PAF(16:1), were both seen to be increasing with follicle diameter. These findings suggest that PAF within FF is increasing as preovulatory follicles approach ovulation. Additionally, immunofluorescence staining identified the PAF Receptor (PAFR) in the luminal pericellular, apical and basal aspect of equine oviductal epithelial cells. Lastly, an equine oviductal epithelial organoid model was generated, and showed that the addition of PAF significantly increased the ciliary beat frequency (CBF) (Hz), an action consistent with a role for PAF in embryo migration. It is proposed that the local action of PAF on the ciliated cells of the oviduct propels both the oocyte and the conceptus towards the uterus. In the mare, it appears that PAF is a contributor during the periconception period, potentially being a mediator in the mechanisms of ovulation and in the dialogue of very early pregnancy.