身体机能低下与多种病理状况下的较高死亡率相关。在这里，我们的目的是确定身体成分和身体表现是否可以成为非小细胞肺癌（NSCLC）患者的预后因素。此外，我们进行了探索性方法来确定 NSCLC 患者的血浆样本是否可以直接影响原代肌细胞的代谢和结构表型。这项前瞻性队列研究包括 55 名转移性 NSCLC 患者和 7 名年龄匹配的对照受试者。评估包括身体表现、身体成分、生活质量和总体生存率。收集了 18 名患者的亚队列血浆样本，用于细胞培养和代谢组学分析的探索性研究。我们观察到，在定时 up-and-go 中表现出色的 NSCLC 患者的生存率较高（320%；p = .007） 、坐立（256%；p = .01）和六分钟步行（323%；p = .002）测试与体能较差的 NSCLC 患者相比。类似分析与身体成分测量没有显着关联 (p > .05)。用身体机能低下的 NSCLC 患者的血浆培养的原代人肌管的耗氧率（-54.2%；p<0.0001）和细胞增殖（-44.9%；p=0.007）受损。一项公正的代谢组学分析揭示了身体机能低下的 NSCLC 患者血浆中差异表达的特定代谢物列表。这些新发现表明，身体机能是 NSCLC 患者总体生存的预后因素，并为可能损害身体机能的循环因素提供了新的见解。骨骼肌代谢。© 2024 Stichting 欧洲临床研究学会期刊基金会。约翰·威利出版

Low physical performance is associated with higher mortality rate in multiple pathological conditions. Here, we aimed to determine whether body composition and physical performance could be prognostic factors in non-small cell lung cancer (NSCLC) patients. Moreover, we performed an exploratory approach to determine whether plasma samples from NSCLC patients could directly affect metabolic and structural phenotypes in primary muscle cells.This prospective cohort study included 55 metastatic NSCLC patients and seven age-matched control subjects. Assessments included physical performance, body composition, quality of life and overall survival rate. Plasma samples from a sub cohort of 18 patients were collected for exploratory studies in cell culture and metabolomic analysis.We observed a higher survival rate in NSCLC patients with high performance in the timed up-and-go (+320%; p = .007), sit-to-stand (+256%; p = .01) and six-minute walking (+323%; p = .002) tests when compared to NSCLC patients with low physical performance. There was no significant association for similar analysis with body composition measurements (p > .05). Primary human myotubes incubated with plasma from NSCLC patients with low physical performance had impaired oxygen consumption rate (-54.2%; p < .0001) and cell proliferation (-44.9%; p = .007). An unbiased metabolomic analysis revealed a list of specific metabolites differentially expressed in the plasma of NSCLC patients with low physical performance.These novel findings indicate that physical performance is a prognostic factor for overall survival in NSCLC patients and provide novel insights into circulating factors that could impair skeletal muscle metabolism.© 2024 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd.