研究动态
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Leptolyngbya sp. 藻蓝蛋白粗提取物生物合成硒纳米粒子的生物活性。 SSI24 利用制糖业回收的滤饼废料进行种植。

Bioactivity of selenium nanoparticles biosynthesized by crude phycocyanin extract of Leptolyngbya sp. SSI24 cultivated on recycled filter cake wastes from sugar-industry.

发表日期:2024 Jul 26
作者: Sara Saad, Amr Mohamed Abdelghany, Ghada Samir Abou-ElWafa, Heshmat Soliman Aldesuquy, Eladl Eltanahy
来源: Microbial Cell Factories

摘要:

甜菜滤饼(BFC)是制糖工业产生的食品级固体废物,构成永久性污染源。蓝藻被认为是各种生物活性化合物(例如藻蓝蛋白色素)的可持续资源,具有有价值的应用。本研究旨在使用甜菜滤饼提取物(BFCE)作为经济培养蓝藻 Leptolyngbya sp 的替代培养基。 SSI24 PP723083,再生物精制出藻蓝蛋白色素等生物活性成分,可用于生产硒纳米粒子。批量实验结果显示,BG11培养基中蛋白质含量最高(47.9%);然而,最大碳水化合物和脂质含量为 25% BFCE(分别为 15.25 和 10.23%)。此外,75% BFCE 培养基刺激藻蓝蛋白含量 (25.29 mg/g),与 BG11 (22.8 mg/g) 相比变化不显着。此外,在 BG11 和 75% BFCE 上培养的 Leptolyngbya sp SSI24 的藻蓝蛋白粗提取物成功地生产了两种提取物中平均尺寸分​​别为 95 和 96 nm 的球形硒纳米粒子 (Se-NP)。此外,XRD结果表明生物合成的Se-NPs具有结晶性质。此外,生物合成的 Se-NPs 在对照和 75% BFCE 处理中的 Zeta 电位分别等于 - 17 mV 和 - 15.03 mV,表明其稳定性。生物合成的 Se-NPs 对革兰氏阳性菌的有效性高于对革兰氏阴性菌的有效性。此外,与 75% BFCE 培养基相比,BG11 生物合成的 Se-NP 具有更高的抗氧化活性,IC50 为 60±0.7。此外,从在 75% BFCE 上培养的 Leptolyngbya sp 中提取的藻蓝蛋白生物合成的 Se-NPs 对人乳腺癌细胞系表现出很强的抗癌活性,IC50 为 17.31±0.63 µg/ml。添加 BFCE 的培养基可用于培养用于积累藻蓝蛋白的蓝藻菌株,用于绿色合成具有生物应用的硒纳米颗粒。© 2024。作者。
Beet filter cake (BFC) is a food-grade solid waste produced by the sugar industry, constituting a permanent source of pollution. Cyanobacteria are considered a sustainable resource for various bioactive compounds such as phycocyanin pigment with valuable applications. This study aimed to use beet filter cake extract (BFCE) as an alternative medium for the economic cultivation of cyanobacterium Leptolyngbya sp. SSI24 PP723083, then biorefined the bioactive component such as phycocyanin pigment that could be used in the production of selenium nanoparticles.The results of the batch experiment displayed that the highest protein content was in BG11medium (47.9%); however, the maximum carbohydrate and lipid content were in 25% BFCE (15.25 and 10.23%, respectively). In addition, 75% BFCE medium stimulated the phycocyanin content (25.29 mg/g) with an insignificant variation compared to BG11 (22.8 mg/g). Moreover, crude phycocyanin extract from Leptolyngbya sp SSI24 cultivated on BG11 and 75% BFCE successfully produced spherical-shaped selenium nanoparticles (Se-NPs) with mean sizes of 95 and 96 nm in both extracts, respectively. Moreover, XRD results demonstrated that the biosynthesized Se-NPs have a crystalline nature. In addition, the Zeta potential of the biosynthesized Se-NPs equals - 17 mV and - 15.03 mV in the control and 75% BFCE treatment, respectively, indicating their stability. The biosynthesized Se-NPs exhibited higher effectiveness against Gram-positive bacteria than Gram-negative bacteria. Moreover, the biosynthesized Se-NPs from BG11 had higher antioxidant activity with IC50 of 60 ± 0.7 compared to 75% BFCE medium. Further, Se-NPs biosynthesized from phycocyanin extracted from Leptolyngbya sp cultivated on 75% BFCE exhibited strong anticancer activity with IC50 of 17.31 ± 0.63 µg/ml against the human breast cancer cell line.The BFCE-supplemented medium can be used for the cultivation of cyanobacterial strain for the phycocyanin accumulation that is used for the green synthesis of selenium nanoparticles that have biological applications.© 2024. The Author(s).