三维 (3D) 细胞培养技术在活力和功能上优于 2D 方法，正用于开发创新的癌症疫苗。肿瘤球体在结构和功能上与实际肿瘤相似，可以使用 3D 细胞培养来开发。这些球状疫苗显示出比 2D 细胞疫苗更优异的抗肿瘤免疫反应。使用 3D 细胞培养也可以更有效地生产树突状细胞疫苗。个性化癌症疫苗正在使用 3D 细胞培养进行开发，与 2D 方法相比具有显着的优势。 3D 细胞培养的更自然条件可能会促进 2D 培养中未表达的肿瘤抗原的表达，从而有可能通过将肿瘤细胞与其他细胞类型共培养来生产更有针对性的疫苗。使用 3D 细胞培养的先进癌症疫苗预计很快就会问世。版权所有 © 2024 Elsevier Ltd。保留所有权利。

Three-dimensional (3D) cell culture techniques, which are superior to 2D methods in viability and functionality, are being used to develop innovative cancer vaccines. Tumor spheroids, which are structurally and functionally similar to actual tumors, can be developed using 3D cell culture. These spheroid vaccines have shown superior antitumor immune responses to 2D cell-based vaccines. Dendritic cell vaccines can also be produced more efficiently using 3D cell culture. Personalized cancer vaccines are being developed using 3D cell culture, providing substantial benefits over 2D methods. The more natural conditions of 3D cell culture might promote the expression of tumor antigens not expressed in 2D culture, potentially allowing for more targeted vaccines by co-culturing tumor cells with other cell types. Advanced cancer vaccines using 3D cell cultures are expected soon.Copyright © 2024 Elsevier Ltd. All rights reserved.