CRISPR 技术的进步改变了实验研究的游戏规则，彻底改变了生命科学的各个领域，更深刻的是癌症研究。细胞死亡途径是癌细胞中最不受管制的途径之一，被认为是癌症发展的关键方面。几十年来，由于尖端技术的革命，我们对程序性细胞死亡机制的了解大幅增加。 CRISPR 系统的出现扩展了可用的筛选平台和基因组工程工具箱，以检测突变并创建精确的基因组编辑。在这种情况下，该系统精确识别和靶向导致癌症发展和治疗耐药的细胞死亡信号通路突变的能力，是转变和加速个体化癌症治疗的有利选择。个性化癌症治疗的概念基于识别个体肿瘤及其微环境的分子特征，以便提供具有最高可能结果和最小毒性的精确治疗。这项研究通过识别和靶向特定的细胞死亡途径，探索了 CRISPR 技术在精准癌症治疗中的潜力。它显示了 CRISPR 在寻找程序性细胞死亡中涉及的关键成分和突变方面的前景，使其成为靶向癌症治疗的潜在工具。然而，这项研究也强调了未来研究中需要解决的挑战和局限性，以充分发挥 CRISPR 在癌症治疗中的潜力。© 2024。作者。

Advancements in the CRISPR technology, a game-changer in experimental research, have revolutionized various fields of life sciences and more profoundly, cancer research. Cell death pathways are among the most deregulated in cancer cells and are considered as critical aspects in cancer development. Through decades, our knowledge of the mechanisms orchestrating programmed cellular death has increased substantially, attributed to the revolution of cutting-edge technologies. The heroic appearance of CRISPR systems have expanded the available screening platform and genome engineering toolbox to detect mutations and create precise genome edits. In that context, the precise ability of this system for identification and targeting of mutations in cell death signaling pathways that result in cancer development and therapy resistance is an auspicious choice to transform and accelerate the individualized cancer therapy. The concept of personalized cancer therapy stands on the identification of molecular characterization of the individual tumor and its microenvironment in order to provide a precise treatment with the highest possible outcome and minimum toxicity. This study explored the potential of CRISPR technology in precision cancer treatment by identifying and targeting specific cell death pathways. It showed the promise of CRISPR in finding key components and mutations involved in programmed cell death, making it a potential tool for targeted cancer therapy. However, this study also highlighted the challenges and limitations that need to be addressed in future research to fully realize the potential of CRISPR in cancer treatment.© 2024. The Author(s).