癌症相关成纤维细胞（CAF）是肿瘤微环境的主要组成部分，与肿瘤的增殖、转移、复发和耐药相关。随着测序技术的发展，单细胞RNA测序已成为识别肿瘤微环境中CAF的流行方法。尽管 CAF 的缺点（例如缺乏空间景观）仍然存在，但最近的研究利用空间转录组学与单细胞 RNA 测序相结合来解决这个问题。这些多组学分析可以解决空间转录组学中的单细胞分辨率问题。在这篇综述中，我们总结了有关 CAF 靶向解决肿瘤组织中的耐药性、血管生成、代谢重编程和转移问题的最新文献。© 2024。作者。

Cancer-associated fibroblasts (CAFs) are the major components of the tumor microenvironment and are related to tumor proliferation, metastasis, relapse, and drug resistance. With the development of sequencing technologies, single-cell RNA sequencing has become a popular method for identifying CAFs in the tumor microenvironment. Whereas the drawbacks of CAFs, such as the lack of a spatial landscape, still exist, recent research has utilized spatial transcriptomics combined with single-cell RNA sequencing to address this issue. These multiomics analyses can resolve the single-cell resolution problem in spatial transcriptomics. In this review, we summarized the recent literature regarding the targeting of CAFs to address drug resistance, angiogenesis, metabolic reprogramming and metastasis in tumor tissue.© 2024. The Author(s).