TMPRSS2:ERG 基因融合负向调节前列腺腺癌细胞系中的 PSMA 表达。因此，我们研究了原发性 PCa 根治性前列腺切除术 (RPE) 标本中的免疫组织化学 (IHC) ERG 表达（潜在 ERG 重排的替代物）和 PSMA 表达模式，包括相应的 PSMA-PET 扫描。两组 RPE 样本（总 n= 148)：在具有可用 RPE 和术前 [68Ga]Ga-PSMA-11 PET、WHO/ISUP 分级组的队列 #1（n=62 名患者）中，IHC-ERG（阳性与阴性）和 IHC-PSMA 表达（% PSMA 阴性肿瘤面积 (PSMA%neg) 与相应的 SUVmax 相关。在仅包括 RPE 的第二队列 #2（n = 86 名患者）中，评估了相同的组织病理学参数。队列 #1：具有 IHC-ERG 表达的 PCa（35.5%）显示出显着较低的 IHC-PSMA 表达和较低的 SUVmax 值。 PET 扫描。 9 例 PSMA-PET 扫描阴性的 PCa 中有 8 例 IHC-ERG 呈阳性，并证实了 TMPRSS2::ERG 重排。在 IHC-PSMA 阳性 PCa 中，IHC-ERG 阳性与较低的 SUVmax 值显着相关。在队列 #2 中，IHC-ERG 阴性 PCa 中仅 0-10% PSMA%neg 肿瘤区域证实了更高的 IHC-PSMA%neg 和 IHC-ERG 表达。IHC-ERG 表达与更多异质性和在两个独立的 RPE 队列中 IHC-PSMA 组织表达较低。 RPE 组织中的 ERG 阳性与相应 PET 扫描中较低的 [68Ga]Ga-PSMA-11 摄取有很强的相关性。结果可作为未来生物标志物开发的基础，以实现针对肿瘤的个性化成像方法。© 2024。作者。

TMPRSS2:ERG gene fusion negatively regulates PSMA expression in prostate adenocarcinoma (PCa) cell lines. Therefore, immunohistochemical (IHC) ERG expression, a surrogate for an underlying ERG rearrangement, and PSMA expression patterns in radical prostatectomy (RPE) specimens of primary PCa, including corresponding PSMA-PET scans were investigated.Two cohorts of RPE samples (total n=148): In cohort #1 (n=62 patients) with available RPE and preoperative [68Ga]Ga-PSMA-11 PET, WHO/ISUP grade groups, IHC-ERG (positive vs. negative) and IHC-PSMA expression (% PSMA-negative tumour area, PSMA%neg) were correlated with the corresponding SUVmax. In the second cohort #2 (n=86 patients) including RPE only, same histopathological parameters were evaluated.Cohort #1: PCa with IHC-ERG expression (35.5%) showed significantly lower IHC-PSMA expression and lower SUVmax values on the corresponding PET scans. Eight of 9 PCa with negative PSMA-PET scans had IHC-ERG positivity, and confirmed TMPRSS2::ERG rearrangement. In IHC-PSMA positive PCa, IHC-ERG positivity was significantly associated with lower SUVmax values. In cohort #2, findings of higher IHC-PSMA%neg and IHC-ERG expression was confirmed with only 0-10% PSMA%neg tumour areas in IHC-ERG-negative PCa.IHC-ERG expression is significantly associated with more heterogeneous and lower IHC-PSMA tissue expression in two independent RPE cohorts. There is a strong association of ERG positivity in RPE tissue with lower [68Ga]Ga-PSMA-11 uptake on corresponding PET scans. Results may serve as a base for future biomarker development to enable tumour-tailored, individualized imaging approaches.© 2024. The Author(s).