神经母细胞瘤是一种常见的儿童肿瘤，对治疗干预提出了重大挑战，特别是对于高危病例。本研究旨在通过研究短期和长期脉冲磁场暴露对油酸 (OA) 治疗的神经母细胞瘤体外模型中神经元凋亡机制的潜在分子影响，填补我们对神经母细胞瘤治疗理解的一个关键空白.培养细胞并分为以下六个实验组：（I）未处理组（NT）； (II) OA治疗组(OA)； (III) 暴露于脉冲磁场15分钟(15分钟PEMF  OA)后用OA处理的组； (IV)脉冲磁场暴露12小时后用OA处理的组(12小时PEMF OA)； (V) 暴露于脉冲磁场 15 分钟（15 分钟 PEMF）的组； (VI) 暴露于脉冲磁场 12 小时 (12 小时 PEMF) 的组。评估了细胞活力、细胞凋亡率以及关键凋亡基因（TP53、Bcl2、Bax 和 Caspase-3）的 mRNA 水平。观察到细胞活力显着降低，特别是在长期脉冲磁力后接受 OA 治疗的组中现场曝光。流式细胞术显示细胞凋亡率升高，尤其是在细胞凋亡的早期阶段。 qRT-PCR 分析表明，长期脉冲磁场暴露后，经 OA 处理的细胞中裂解的 Caspase-3、Bax/Bcl2 比率和 TP53 的表达增加，表明细胞凋亡途径增强。研究结果表明，长期脉冲磁场暴露和 OA 治疗表现出潜在的协同作用，导致 SH-SY5Y 细胞凋亡的诱导。我们得出的结论是，脉冲磁场刺激有潜力作为基于油酸的神经母细胞瘤治疗的辅助疗法。

Neuroblastoma, a prevalent childhood tumor, poses significant challenges in therapeutic interventions, especially for high-risk cases. This study aims to fill a crucial gap in our understanding of neuroblastoma treatment by investigating the potential molecular impacts of short- and long-term pulsed magnetic field exposure on the neuronal apoptosis mechanism in an in vitro model of neuroblastoma treated with oleic acid (OA).Cells were cultured and divided into six following experimental groups: (I) Nontreated group (NT); (II) OA-treated group (OA); (III) Group treated with OA after being exposed to the pulsed magnetic field for 15-min (15 min PEMF + OA); (IV) Group treated with OA after being exposed to the pulsed magnetic field for 12 h (12 h PEMF + OA); (V) Group exposed to the pulsed magnetic field for 15 min (15 min PEMF); and (VI) Group exposed to the pulsed magnetic field for 12 h (12 h PEMF). Cell viability, rates of apoptosis, and mRNA levels of key apoptotic genes (TP53, Bcl2, Bax, and Caspase-3) were assessed.Significant reductions in cell viability were observed, particularly in the group treated with OA following long-term pulsed magnetic field exposure. Flow cytometry revealed elevated apoptosis rates, notably in the early stages of apoptosis. qRT-PCR analysis demonstrated increased expression of cleaved Caspase-3, Bax/Bcl2 ratio, and TP53 in cells treated with OA following long-term pulsed magnetic field exposure, signifying enhanced apoptotic pathways.The findings indicate that long-term pulsed magnetic field exposure and OA treatment exhibit potential synergistic effects leading to the induction of apoptosis in SH-SY5Y cells. We have concluded that stimulations of pulsed magnetic field have the potential to serve as an adjuvant therapy for oleic acid-based treatment of neuroblastoma.