心脏粘液瘤是心脏内常见的肿瘤，有可能危及生命。然而，这种情况的细胞组成仍不清楚。为了填补这一空白，我们基于单细胞测序分析了来自心脏粘液瘤组织的 75,641 个细胞。我们定义了一群粘液瘤细胞，它们与成纤维细胞相似，但它们的区别在于磷酸二酯酶和与细胞增殖、分化和粘附相关的基因表达增加。细胞群的临床相关性表明粘液瘤细胞和 M2 样巨噬细胞浸润的比例较高，以及它们增强的空间相互作用，被发现对栓塞的发生有显着影响。粘液瘤周围的免疫细胞表现出抑制特征，T细胞功能受损，其特征是表达GZMK和TOX，以及表达PDGFC等生长因子的促肿瘤巨噬细胞的大量浸润。此外，体外共培养实验表明巨噬细胞显着促进粘液瘤细胞的生长。总之，本研究提出了心脏粘液瘤的综合单细胞图谱，强调了粘液瘤细胞的异质性及其对免疫细胞的协同影响。这些发现揭示了心脏粘液瘤复杂的病理学，并提出了潜在的干预目标。© 2024。作者。

Cardiac myxoma is a commonly encountered tumor within the heart that has the potential to be life-threatening. However, the cellular composition of this condition is still not well understood. To fill this gap, we analyzed 75,641 cells from cardiac myxoma tissues based on single-cell sequencing. We defined a population of myxoma cells, which exhibited a resemblance to fibroblasts, yet they were distinguished by an increased expression of phosphodiesterases and genes associated with cell proliferation, differentiation, and adhesion. The clinical relevance of the cell populations indicated a higher proportion of myxoma cells and M2-like macrophage infiltration, along with their enhanced spatial interaction, were found to significantly contribute to the occurrence of embolism. The immune cells surrounding the myxoma exhibit inhibitory characteristics, with impaired function of T cells characterized by the expression of GZMK and TOX, along with a substantial infiltration of tumor-promoting macrophages expressed growth factors such as PDGFC. Furthermore, in vitro co-culture experiments showed that macrophages promoted the growth of myxoma cells significantly. In summary, this study presents a comprehensive single-cell atlas of cardiac myxoma, highlighting the heterogeneity of myxoma cells and their collaborative impact on immune cells. These findings shed light on the complex pathobiology of cardiac myxoma and present potential targets for intervention.© 2024. The Author(s).