β-紫罗兰酮是一种去甲异戊二烯类化合物，是一种从植物中提取的天然芳香族化合物，具有抗癌、抗氧化和驱虫等多种生物活性。由于热带假丝酵母生物量大、环境耐受性强，因此选择非常规产油酵母来高效合成β-紫罗兰酮。我们最初研究了细胞质和亚细胞区室独立合成 β-紫罗兰酮的能力。随后，通过酶的适应性筛选、亚细胞定位信号肽的功能鉴定以及亚细胞区室组合策略，获得了152.4 mg/L的β-紫罗兰酮滴度。最后，进行限速酶的定向进化和关键酶的过度表达以提高β-紫罗兰酮的产量。摇瓶中的滴度为 400.5 mg/L，生物反应器中的滴度为 730 mg/L。这项研究首次在热带念珠菌中从头合成了 β-紫罗兰酮，为萜类香料提供了一种新颖的细胞基础，具有巨大的工业潜力。

β-ionone, a norisoprenoid, is a natural aromatic compound derived from plants, which displays various biological activities including anticancer, antioxidant and deworming properties. Due to its large biomass and strong environmental tolerance, the nonconventional oleaginous yeast Candida tropicalis was selected to efficiently synthesize β-ionone. We initially investigated the capacity of the cytoplasm and subcellular compartments to synthesize β-ionone independently. Subsequently, through adaptive screening of enzymes, functional identification of subcellular localization signal peptides and subcellular compartment combination strategies, a titer of 152.4 mg/L of β-ionone was achieved. Finally, directed evolution of rate-limiting enzyme and overexpression of key enzymes were performed to enhance β-ionone production. The resulting titer was 400.5 mg/L in shake flasks and 730 mg/L in a bioreactor. This study demonstrates the first de novo synthesis of β-ionone in C. tropicalis, providing a novel cellular chassis for terpenoid fragrances with considerable industrial potential.