结直肠癌（CRC）是一种广泛存在的恶性肿瘤，其发病机制复杂且尚未完全阐明。本研究旨在探讨双歧杆菌在尿素循环（UC）中的作用及其对结直肠癌进展的影响，这是一个以前没有广泛研究的课题。本研究利用生物信息学和实验方法，分析结直肠癌患者的细菌丰度进行比较给健康的人。该研究特别关注 BA 的丰度。此外，还进行了转录组数据分析和细胞实验，以研究双歧杆菌对氨代谢和线粒体功能的影响，特别检查其对关键 UC 基因 ALB 的调节。分析显示，CRC 患者中双歧杆菌丰度显着下降。此外，双歧杆菌被发现可以通过调节 ALB 基因来抑制氨代谢并诱导线粒体功能障碍，这对于 UC 至关重要。这些影响有助于抑制结直肠癌细胞增殖，动物实验结果证实了这一发现。本研究阐明了双歧杆菌影响结直肠癌进展的分子机制，强调了其在调节关键代谢途径中的作用。这些发现为 CRC 治疗的新治疗策略提供了潜在目标，强调了微生物群在癌症进展中的重要性。© 2024。作者。

Colorectal cancer (CRC) is a widespread malignancy with a complex and not entirely elucidated pathogenesis. This study aims to explore the role of Bifidobacterium in the urea cycle (UC) and its influence on the progression of CRC, a topic not extensively studied previously.Utilizing both bioinformatics and experimental methodologies, this research involved analyzing bacterial abundance in CRC patients in comparison to healthy individuals. The study particularly focused on the abundance of BA. Additionally, transcriptomic data analysis and cellular experiments were conducted to investigate the impact of Bifidobacterium on ammonia metabolism and mitochondrial function, specifically examining its regulation of the key UC gene, ALB.The analysis revealed a significant decrease in Bifidobacterium abundance in CRC patients. Furthermore, Bifidobacterium was found to suppress ammonia metabolism and induce mitochondrial dysfunction through the regulation of the ALB gene, which is essential in the context of UC. These impacts contributed to the suppression of CRC cell proliferation, a finding corroborated by animal experimental results.This study elucidates the molecular mechanism by which Bifidobacterium impacts CRC progression, highlighting its role in regulating key metabolic pathways. These findings provide potential targets for novel therapeutic strategies in CRC treatment, emphasizing the importance of microbiota in cancer progression.© 2024. The Author(s).