评估组织分子扩散的光：病理学的歧视
Light in evaluation of molecular diffusion in tissues: Discrimination of pathologies
影响因子:17.60000
分区:医学1区 Top / 药学1区
发表日期:2024 Sep
作者:
Luís R Oliveira, Maria R Pinheiro, Daria K Tuchina, Polina A Timoshina, Maria I Carvalho, Luís M Oliveira
摘要
对组织中不同分子的扩散特性的评估是在各个领域中引起人们感兴趣的主题，例如皮肤病学/美容，临床医学，植入学和食物保存。在这篇综述中，对使用动力学光谱测量的最新研究的讨论进行了评估在各种组织中的这种扩散特性。通过将离体组织浸入代理中或通过在体内局部应用这些代理，可以通过动力学的准直发或弥漫性反射率光谱来评估它们的扩散特性。使用这种方法，最近的研究能够区分健康和患病组织之间的药物的扩散特性，尤其是在癌症和糖尿病的情况下。就癌症而言，还可以评估从健康到癌症结肠直肠和肾脏组织的移动水含量增加5％。考虑到某些代理在生物或食品中的应用以保护它们免受低温保存（冷冻保存）的恶化，并且知道可能会逆转这种药物的纳入，因此还讨论了这些领域的一些研究。考虑到该方法可以获取的宽带应用宽带的光谱评估以及该方法可以获取的生理诊断数据的扩散性能，有关果实甜度或对毒药扩散在组织或反派剂应用中用于治疗优化目的的毒物扩散目的的进一步研究被视为未来的观点。
Abstract
The evaluation of the diffusion properties of different molecules in tissues is a subject of great interest in various fields, such as dermatology/cosmetology, clinical medicine, implantology and food preservation. In this review, a discussion of recent studies that used kinetic spectroscopy measurements to evaluate such diffusion properties in various tissues is made. By immersing ex vivo tissues in agents or by topical application of those agents in vivo, their diffusion properties can be evaluated by kinetic collimated transmittance or diffuse reflectance spectroscopy. Using this method, recent studies were able to discriminate the diffusion properties of agents between healthy and diseased tissues, especially in the cases of cancer and diabetes mellitus. In the case of cancer, it was also possible to evaluate an increase of 5% in the mobile water content from the healthy to the cancerous colorectal and kidney tissues. Considering the application of some agents to living organisms or food products to protect them from deterioration during low temperature preservation (cryopreservation), and knowing that such agent inclusion may be reversed, some studies in these fields are also discussed. Considering the broadband application of the optical spectroscopy evaluation of the diffusion properties of agents in tissues and the physiological diagnostic data that such method can acquire, further studies concerning the optimization of fruit sweetness or evaluation of poison diffusion in tissues or antidote application for treatment optimization purposes are indicated as future perspectives.