各种细胞类型释放的细胞外囊泡 (EV) 已被证明可以介导生物活性有效负载从供体细胞向受体细胞的传递，然而，目前尚不清楚这些 EVs 来自哪些细胞类型。我们重点关注荧光报告基因来监测 EV 的释放，特别是在细胞类型特异性启动子控制下的 EV 释放，我们解决了培养细胞、正常组织以及发育、损伤、癌症和伤口愈合模型中遗传工具的翻译相关性众所周知，体内许多类型的细胞通过质膜上的融合和释放过程释放EV。由于对于通过受调节的内体运输途径与非特异性机制释放多少比例的 EV 仍存在争议，因此新型分子工具的开发和验证对于解决 EV 的细胞来源非常重要。需要开发和表征新细胞类型特异性报告小鼠模型建立在此处详述的示例的基础上，用于识别 EV 的细胞来源，遗传方法可用于解决这些关键限制。报告系统的进步将推动对 EV 子集的更好理解，以识别隔室特异性 EV 定位指导伤口愈合领域开发更具转化相关性的模型。

Release of extracellular vesicles (EVs) by various cell types has been shown to mediate the delivery of biologically active payloads from donor cells to recipient cells, however, it remains unclear what cell types these EVs come from. With a focus on fluorescent reporters to monitor the release of EVs, especially those under the control of cell type specific promoters, we address the translational relevance of genetic tools in cultured cells, normal tissues and in models of development, injury, cancer and wound healing.It is well established that EVs are released by many cell types in the body via fusion and release processes at the plasma membrane. Since there remains debate about what fraction of EVs are released through regulated endosomal trafficking pathways vs. non-specific mechanisms the development and validation of novel molecular tools are important to address the cellular source of EVs.There is a need to develop and characterize new cell type-specific reporter mouse models that build upon the examples detailed here to identify the cellular source of EVs with genetic approaches being useful in addressing these critical limitations.Advances in reporter systems will drive a better understanding of EV subsets to identify compartment specific EV localization to guide the development of more translationally relevant models for the wound healing field.