超声刺激微泡（USMB）疗法已被证明能够有效靶向肿瘤血管系统并增强肿瘤异种移植物的辐射效果。在这项研究中，我们研究了这种治疗是否增强了宫颈癌对辐射的敏感性。人类宫颈癌（ME-180和SiHa）细胞经过USMB处理或暴露于辐射（0、2、4、6和8 Gy）或辐射 (8 Gy) 与 USMB 结合。采用克隆形成实验和CCK-8实验分析细胞的增殖能力。分别使用流式细胞术和γ-H2AX（γ-H2AX）免疫荧光染色检测细胞凋亡和DNA双链断裂。进行基质胶小管形成以评估人脐静脉内皮细胞的血管生成。在SiHa细胞异种移植模型中，免疫组化检测肿瘤组织CD31的表达。USMB和放射线协同抑制ME-180和SiHa细胞的生长。 USMB 通过提高促凋亡蛋白的水平来促进辐射诱导的细胞凋亡。此外，USMB 增强了辐射诱导的 γ-H2AX 焦点，从而诱导宫颈癌细胞中的 DNA 双链断裂。 USMB 与辐射相结合可降低体外内皮细胞的血管生成能力。此外，USMB增强了辐射对异种移植模型中肿瘤生长和血管生成的抑制作用。综上所述，USMB暴露有效增强了辐射对宫颈癌的破坏作用，表明USMB可能是一种有前途的宫颈癌放疗增敏剂。

Ultrasound-stimulated microbubble (USMB) therapy has proven efficacy of targeting tumor vasculature and enhancing the effect of radiation in tumor xenografts. In this investigation, we studied whether this treatment enhances the sensitivity of cervical cancer to radiation.Human cervical cancer (ME-180 and SiHa) cells were treated with USMB or exposed to radiation (0, 2, 4, 6 and 8 Gy) or radiation (8 Gy) in combination with USMB. Clone formation assay and CCK-8 assay were used to analyze the proliferation capacity of cells. Apoptosis and DNA double-strand breaks were detected using flow cytometry and immunofluorescence staining of gamma-H2AX (γ-H2AX), respectively. Matrigel tubule formation was performed to evaluate the angiogenesis of human umbilical vein endothelial cells. In xenograft model of SiHa cells, tumor tissue expression of CD31 was detected by immunohistochemistry.USMB and radiation synergistically restrained the growth of ME-180 and SiHa cells. USMB promoted radiation-induced apoptosis by enhancing the levels of proapoptotic proteins. Furthermore, USMB enhanced radiation-induced γ-H2AX foci to induce DNA double-strand breaks in cervical cancer cells. USMB in combination with radiation reduced the angiogenic capacity of endothelial cells in vitro. Moreover, USMB strengthened the inhibitory effect of radiation on tumor growth and angiogenesis in xenograft models.In conclusion, USMB exposure effectively enhanced the destructive effect of radiation on cervical cancer, suggesting that USMB might be a promising sensitizer of radiotherapy to treat cervical cancer.