越来越多的证据表明肿瘤内微生物组在多种实体瘤中发挥着至关重要的作用。然而，妇科恶性肿瘤中的瘤内微生物组在很大程度上尚不清楚。本研究共纳入90例汉族患者，其中宫颈癌、卵巢癌、子宫内膜癌各30例，通过16S rDNA扩增子高通量测序评估瘤内微生物组组成。我们发现所有三种癌症类型中瘤内微生物组的多样性和代谢潜力非常相似。此外，所有三种癌症类型都有一些共同具有较高相对丰度和阳性率的分类群，包括假单胞菌属、丛毛单胞菌属。 sp.、Bradyrhizobium sp.、Saccharomonospora sp.、痤疮皮肤杆菌、Rubrobacter sp.、Dialister micraerophilus 和大肠杆菌。此外，副流感嗜血杆菌和副球菌属。在宫颈癌中，Pelomonas sp。 LDA 鉴定卵巢癌中的粪肠球菌和子宫内膜癌中的粪肠球菌为代表性菌株。此外，在宫颈癌患者中，甲胎蛋白（AFP）（相关系数 = -0.3714）与红杆菌属呈负相关（r = 0.4，95% CI：0.03 至 0.7）。 CA199（相关系数 = 0.3955）与糖单孢菌属呈正相关（r = 0.4，95% CI：0.03 至 0.7）。在卵巢癌患者中，CA125（相关系数 = -0.4451）呈负相关（r = -0.4） ，95% CI：-0.7 至 -0.09）与卟啉单胞菌属。在子宫内膜癌患者中，CEA（相关系数 = -0.3868）与皮肤杆菌呈负相关（r = -0.4，95% CI：-0.7 至 -0.02）痤疮。这项研究促进了我们对妇科恶性肿瘤肿瘤内微生物组的理解。重要性在这项研究中，我们发现妇科恶性肿瘤中肿瘤微生物的组成谱在很大程度上相似，因为共享一些分类单元，并因独特拥有的大量物种而有所不同。某些物种（大多数未报告）被确定与临床特征相关。这项研究促进了我们对妇科恶性肿瘤的了解，并为肿瘤微生物影响女性生殖系统癌症中的肿瘤生物学提供了证据。

Growing evidence have indicated the crucial role of intratumor microbiome in a variety of solid tumor. However, the intratumoral microbiome in gynecological malignancies is largely unknown. In the present study, a total of 90 Han patients, including 30 patients with cancer in cervix, ovary, and endometrium each were enrolled, the composition of intratumoral microbiome was assessed by 16S rDNA amplicon high throughput sequencing. We found that the diversity and metabolic potential of intratumoral microbiome in all three cancer types were very similar. Furthermore, all three cancer types shared a few taxa that collectively take up high relative abundance and positive rate, including Pseudomonas sp., Comamonadaceae gen. sp., Bradyrhizobium sp., Saccharomonospora sp., Cutibacterium acnes, Rubrobacter sp., Dialister micraerophilus, and Escherichia coli. Additionally, Haemophilus parainfluenzae and Paracoccus sp. in cervical cancer, Pelomonas sp. in ovarian cancer, and Enterococcus faecalis in endometrial cancer were identified by LDA to be a representative bacterial strain. In addition, in cervical cancer patients, alpha-fetoprotein (AFP) (correlation coefficient = -0.3714) was negatively correlated (r = 0.4, 95% CI: 0.03 to 0.7) with Rubrobacter sp. and CA199 (correlation coefficient = 0.3955) was positively associated (r = 0.4, 95% CI: 0.03 to 0.7) with Saccharomonospora sp.. In ovarian cancer patients, CA125 (correlation coefficient = -0.4451) was negatively correlated (r = -0.4, 95% CI: -0.7 to -0.09) with Porphyromonas sp.. In endometrial cancer patients, CEA (correlation coefficient = -0.3868) was negatively correlated (r = -0.4, 95% CI: -0.7 to -0.02) with Cutibacterium acnes. This study promoted our understanding of the intratumoral microbiome in gynecological malignancies.IMPORTANCEIn this study, we found the compositional spectrum of tumor microbes among gynecological malignancies were largely similar by sharing a few taxa and differentiated by substantial species owned uniquely. Certain species, mostly unreported, were identified to be associated with clinical characteristics. This study prompted our understanding of gynecological malignancies and offered evidence for tumor microbes affecting tumor biology among cancers in the female reproductive system.