上皮性卵巢癌（EOC）是一种常常致命的恶性肿瘤，其特点是对铂类化疗产生耐药性。因此，准确预测铂类药物疗效对于战略性选择术后干预措施以减轻与次优治疗结果和不良反应相关的风险至关重要。与血浆中的对应物相比，组织源性细胞外囊泡 (tsEV) 已成为检查 EOC 组织独特属性的强大工具。在这项研究中，对从 58 名铂类敏感和 30 名铂类耐药的 EOC 患者获得的 tsEV 进行了 4D 数据独立采集 (DIA) 蛋白质组测序。分析显示差异表达蛋白显着富集，这些蛋白主要与免疫相关途径相关。此外，通过 LASSO 回归鉴定了关键免疫相关蛋白 (IRP)。这些因素与通过单变量逻辑回归选择的临床参数相结合，用于构建采用多元逻辑回归的模型。该模型整合了三个 tsEV IRP：CCR1、IGHV_35 和 CD72，以及一个临床参数，即术后残留病灶的存在。因此，该模型可以预测 EOC 患者术后初始铂类化疗的疗效，甚至在化疗开始之前就提供预后见解。© 2024 作者。 《Journal of Extracellular Vesicles》由 Wiley periodicals LLC 代表国际细胞外囊泡学会出版。

Epithelial ovarian cancer (EOC) is an often-fatal malignancy marked by the development of resistance to platinum-based chemotherapy. Thus, accurate prediction of platinum drug efficacy is crucial for strategically selecting postoperative interventions to mitigate the risks associated with suboptimal therapeutic outcomes and adverse effects. Tissue-derived extracellular vesicles (tsEVs), in contrast to their plasma counterparts, have emerged as a powerful tool for examining distinctive attributes of EOC tissues. In this study, 4D data-independent acquisition (DIA) proteomic sequencing was performed on tsEVs obtained from 58 platinum-sensitive and 30 platinum-resistant patients with EOC. The analysis revealed a notable enrichment of differentially expressed proteins that were predominantly associated with immune-related pathways. Moreover, pivotal immune-related proteins (IRPs) were identified by LASSO regression. These factors, combined with clinical parameters selected through univariate logistic regression, were used for the construction of a model employing multivariate logistic regression. This model integrated three tsEV IRPs, CCR1, IGHV_35 and CD72, with one clinical parameter, the presence of postoperative residual lesions. Thus, this model could predict the efficacy of initial platinum-based chemotherapy in patients with EOC post-surgery, providing prognostic insights even before the initiation of chemotherapy.© 2024 The Author(s). Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.