细胞片层技术（CST）主要应用于组织工程中的修复目的。我们的初步研究表明，使用 CST 建立的体内前列腺癌模型优于传统的细胞悬浮方法。然而，CST 用于膀胱癌细胞的潜力尚未得到探索。在这项研究中，我们研究了两种膀胱癌细胞系 T24 和 5637 形成细胞片层的能力。我们发现T24细胞成功形成细胞片层。然后我们进行染色以评估 T24 细胞片的完整性、特异性标记物和增殖特征。我们的研究结果表明，可以建立膀胱癌细胞片层，为体内和体外膀胱癌研究以及患者个性化药物选择提供有价值的工具。© 2024 作者。

Cell sheet technology (CST) has primarily been applied in tissue engineering for repair purposes. Our preliminary research indicates that an in vivo prostate cancer model established using CST outperforms traditional cell suspension methods. However, the potential for CST to be used with bladder cancer cells has not yet been explored. In this study, we investigated the ability of two bladder cancer cell lines, T24 and 5637, to form cell sheets. We found that T24 cells successfully formed cell sheets. We then performed staining to evaluate the integrity, specific markers, and proliferation characteristics of the T24 cell sheets. Our findings demonstrate that bladder cancer cell sheets can be established, providing a valuable tool for both in vivo and in vitro bladder cancer studies and for personalized drug selection for patients.© 2024 The Authors.