环状RNA（circRNA）被认为介导人类癌症的发生和发展，通常充当微小RNA（miRNA）海绵来调节下游基因表达。然而，尚不清楚 circACVR2A (hsa_circ_0001073) 是否以及如何参与 HCC 的进展。本研究的目的是阐明circACVR2A在调节肝细胞癌细胞（HCC）进展中的潜在作用和分子机制。通过定量逆转录聚合酶链反应（RT-PCR）或蛋白质印迹法测定circACVR2A、microRNA（miR511-5p）和PI3K-Akt信号通路中相关蛋白的丰度。分别通过CCK-8、Transwell分析和Tunel染色分析细胞活力、侵袭和凋亡。通过双荧光素酶报告基因测定评估 circACVR2A 和 microRNA 之间的相互作用。结果显示，circACVR2A在肝癌细胞系中高表达。我们的体内和体外数据表明，circACVR2A促进HCC的增殖、迁移和侵袭。在机制方面，我们发现circACVR2A可以直接与miR511-5p相互作用，并作为miRNA海绵调节PI3K-Akt信号通路中相关蛋白的表达。在HCC中，circACVR2A可以介导miR-511-5p/mRNA网络激活PI3K信号通路。这表明以circACVR2A为核心的分子调控网络是肝细胞癌诊断和治疗的新的潜在靶点。©2024。作者。

Circular RNA (circRNA) is thought to mediate the occurrence and development of human cancer and usually acts as a tiny RNA (miRNA) sponge to regulate downstream gene expression. However, it is not clear whether and how circACVR2A (hsa_circ_0001073) is involved in the progression of HCC. The purpose of this study is to clarify the potential role and molecular mechanism of circACVR2A in regulating the progression of hepatocellular carcinoma cells (HCC). The abundance of related proteins in circACVR2A, microRNA (miR511-5p) and PI3K-Akt signaling pathway was determined by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) or Western blotting. Cell viability, invasion and apoptosis were analyzed by CCK-8, Transwell analysis and Tunel staining, respectively. The interaction between circACVR2A and microRNA was evaluated by double luciferase reporter gene assay. The results showed that circACVR2A was highly expressed in hepatocellular carcinoma cell lines. Our in vivo and in vitro data showed that circACVR2A promoted the proliferation, migration and invasion of HCC. In terms of mechanism, we found that circACVR2A can directly interact with miR511-5p and act as a miRNA sponge to regulate the expression of related proteins in PI3K-Akt signaling pathway.In HCC, circACVR2A can mediate miR-511-5p/mRNA network to activate PI3K signal pathway. This shows that the molecular regulatory network with circACVR2A as the core is a new potential target for diagnosis and treatment of hepatocellular carcinoma.© 2024. The Author(s).