皮肤鳞状细胞癌（SCC）是一种常见的恶性肿瘤，脂质代谢失调与其发病机制有关。然而，鳞状细胞癌中脂质变化的详细表征仍然有限。我们使用液相色谱-质谱法 (LC-MS) 分析了 34 名鳞状细胞癌患者和邻近健康组织（距离肿瘤边缘超过 1 厘米）的组织样本中的脂质代谢变化。 。使用主成分分析（PCA）和稀疏偏最小二乘判别分析（sPLS-DA）来识别数据可视化和歧视性脂质谱。使用外部数据集（来自之前的研究，该研究使用脂质组学方法类似地探索了口腔鳞状细胞癌中的脂质谱）鉴定并进一步验证了参与鳞状细胞癌代谢的关键脂质。通过通路富集分析来阐明与这些关键脂质相关的代谢途径。通过比较SCC和健康组织，鉴定出八种脂质，包括PI（16:0/22:4）、PI（18:1/20:4）、PE (16:0/20:4)、PE(16:0/22:5)、PE(16:0/22:6)、PE(18:1/20:3)、PC(18:1/20) :2) 和 PC(18:2/20:2)，经独立数据集证实。所有这些脂质在鳞状细胞癌肿瘤组织中均上调。通路富集分析揭示了甘油磷脂代谢通路的显着改变，特别是影响二酰基甘油磷酸胆碱、甘油磷酸乙醇胺和甘油磷酸肌醇的代谢。我们的研究结果表明，甘油磷脂代谢失调在鳞状细胞癌的发生中起着关键作用。版权所有 © 2024 Mei, Gan, Wang, Xu 、叶和杨。

Skin squamous cell carcinoma (SCC) is a prevalent malignancy, and dysregulated lipid metabolism has been implicated in its pathogenesis. However, detailed characterization of lipid alterations in SCC remains limited.We analyzed lipid metabolic variations in tissue samples from 34 SCC patients and adjacent healthy tissues (located more than 1 cm from the tumor margin) using liquid chromatography-mass spectrometry (LC-MS). Data visualization and discriminatory lipid profiles were identified using principal component analysis (PCA) and sparse partial least squares discriminant analysis (sPLS-DA). Key lipids involved in the SCC metabolism were identified and further validated using an external data set (from a previous study, which similarly explored lipid profiles in oral SCC using lipidomics approaches). Pathway enrichment analysis was conducted to elucidate the metabolic pathways associated with these key lipids.Eight lipids were identified by comparing SCC and healthy tissues including PI(16:0/22:4), PI(18:1/20:4), PE(16:0/20:4), PE(16:0/22:5), PE(16:0/22:6), PE(18:1/20:3), PC(18:1/20:2), and PC(18:2/20:2), as confirmed by independent datasets. All of these lipids were upregulated in SCC tumor tissues. Pathway enrichment analysis revealed significant alterations in glycerophospholipid metabolic pathways, particularly affecting the metabolism of diacylglycerophosphocholines, glycerophosphoethanolamines, and glycerophosphoinositols.Our findings reveal that dysregulated glycerophospholipid metabolism plays a pivotal role in the development of SCC.Copyright © 2024 Mei, Gan, Wang, Xu, Ye and Yang.