冰冻切片（FS）是一种在术中广泛使用的技术，用于提供初步的组织病理学诊断，以便在手术时立即做出决定。我们的目的是研究各种妇科肿瘤样本中快速冷冻诱导的组织抗原性的潜在变化。使用妇科病理学中常用的一组免疫染色剂对总共 177 个 FS 和 177 个非冰冻切片 (NFS) 组织切片进行了测试，包括激素受体（雌激素受体、孕激素受体）、HER2、错配修复蛋白（MSH6、PMS2）、程序性细胞死亡1配体1（PD-L1）、p53、napsin A和ɑ-甲基酰基辅酶-A消旋酶。免疫组织化学结果分为阳性或阴性，随后根据染色的分布和强度对阳性病例进行评分。某些免疫染色，如 HER2、PD-L1 和 p53，根据既定指南进行评分。FS 和 NFS 块之间的总体一致性为 87%；在13%的差异病例中，大多数（10.7%）被归类为轻微病例，只有数量上的差异，没有可预见的临床意义。在 2.3% 的病例中，出现了对疾病管理具有潜在影响的重大质变。我们得出的结论是，在大多数情况下，FS 组织块可以安全地用于免疫组织化学研究，因为大多数差异病例仅显示染色方面的微小差异，没有预期的差异。临床意义。然而，对于某些标记物，包括 HER2、p53 和 PMS2，当该选项可用时，首选 NFS 块。© 作者 2024。由牛津大学出版社代表美国临床病理学会出版。版权所有。如需商业重复使用，请联系 reprints@oup.com 获取转载和转载的翻译权。所有其他权限都可以通过我们网站文章页面上的权限链接通过我们的 RightsLink 服务获得 - 如需了解更多信息，请联系journals.permissions@oup.com。

Frozen section (FS) is a technique widely used intraoperatively to render a preliminary histopathologic diagnosis, allowing for immediate decisions at the time of surgery. We aimed to investigate potential variations in tissue antigenicity induced by rapid freezing in a variety of gynecologic tumor samples.A total of 177 FS and 177 non-frozen section (NFS) tissue slides were tested using a panel of immunostains commonly used in gynecologic pathology, including hormone receptors (estrogen receptor, progesterone receptor), HER2, mismatch repair proteins (MSH6, PMS2), programmed cell death 1 ligand 1 (PD-L1), p53, napsin A, and ɑ-methylacyl coenzyme-A racemase. Immunohistochemistry results were categorized as positive or negative, and positive cases were subsequently scored based on the distribution and intensity of the staining. Certain immunostains, such as HER2, PD-L1, and p53, were scored according to the established guidelines.The overall concordance between FS and NFS blocks was 87%; among the 13% of discrepant cases, most (10.7%) were classified as minor, with only quantitative differences without foreseeable clinical significance. In 2.3% of cases, there were major qualitative changes with potential impact on disease management.We concluded that FS tissue blocks may, in most cases, safely be used for immunohistochemical studies because most discrepant cases showed only minor differences in staining, with no anticipated clinical significance. Nevertheless, for certain markers, including HER2, p53, and PMS2, a NFS block is preferred when that option is available.© The Author(s) 2024. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.