冻结部分与非冷冻截面组织之间的抗原性比较：对妇科病理中常用抗体的免疫组织化学研究
Comparison of antigenicity between frozen section vs non-frozen section tissue blocks: An immunohistochemical study of antibodies commonly used in gynecologic pathology
影响因子:1.90000
分区:医学4区 / 病理学3区
发表日期:2024 Aug 07
作者:
Quratulain Obaid, Mehrdad Nadji, Matthew Schlumbrecht, Andre Pinto
摘要
冷冻部分（FS）是一种术中广泛用于术中用于进行初步组织病理学诊断的技术，可以在手术时立即做出决定。我们旨在研究通过在各种妇科肿瘤样品中快速冻结引起的组织抗原性的潜在变化。总共177 fs和177个非冷冻片段（NFS）组织载玻片，使用在包括妇科病理受体的妇科受体受体的妇科受体中，使用了速生病理受体，搭配者，配备了杂种，蛋白质（MSH6，PMS2），程序性细胞死亡1配体1（PD-L1），p53，Napsin A和-甲基酰基辅酶A种族酶。免疫组织化学结果被归类为阳性或阴性，随后根据染色的分布和强度对阳性病例进行评分。根据已建立的指南对某些免疫抑制剂（例如HER2，PD-L1和P53）进行了评分。FS和NFS块之间的整体一致性为87％；在13％的差异病例中，大多数（10.7％）被归类为次要的，只有定量差异而没有可预见的临床意义。在2.3％的病例中，有重大的定性变化，对疾病管理的潜在影响。我们得出的结论是，在大多数情况下，FS组织块可以安全地用于免疫组织化学研究，因为大多数差异病例在染色方面仅显示出较小的染色差异，没有预期的临床意义。然而，对于某些标记，包括HER2，P53和PMS2，当该选项可用时，首选NFS块。版权所有。有关商业重复使用，请联系reprints@oup.com，以获取转载和翻译权以进行转载。所有其他权限都可以通过我们的restrimlink服务通过我们网站上文章页面上的“权限链接”获得 - 有关更多信息，请联系journals.permissions.permissions@oup.com。
Abstract
Frozen section (FS) is a technique widely used intraoperatively to render a preliminary histopathologic diagnosis, allowing for immediate decisions at the time of surgery. We aimed to investigate potential variations in tissue antigenicity induced by rapid freezing in a variety of gynecologic tumor samples.A total of 177 FS and 177 non-frozen section (NFS) tissue slides were tested using a panel of immunostains commonly used in gynecologic pathology, including hormone receptors (estrogen receptor, progesterone receptor), HER2, mismatch repair proteins (MSH6, PMS2), programmed cell death 1 ligand 1 (PD-L1), p53, napsin A, and ɑ-methylacyl coenzyme-A racemase. Immunohistochemistry results were categorized as positive or negative, and positive cases were subsequently scored based on the distribution and intensity of the staining. Certain immunostains, such as HER2, PD-L1, and p53, were scored according to the established guidelines.The overall concordance between FS and NFS blocks was 87%; among the 13% of discrepant cases, most (10.7%) were classified as minor, with only quantitative differences without foreseeable clinical significance. In 2.3% of cases, there were major qualitative changes with potential impact on disease management.We concluded that FS tissue blocks may, in most cases, safely be used for immunohistochemical studies because most discrepant cases showed only minor differences in staining, with no anticipated clinical significance. Nevertheless, for certain markers, including HER2, p53, and PMS2, a NFS block is preferred when that option is available.© The Author(s) 2024. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.