有关体内细胞衰老实验的最少信息指南。
Guidelines for minimal information on cellular senescence experimentation in vivo.
发表日期：2024 Aug 08
作者：
Mikolaj Ogrodnik, Juan Carlos Acosta, Peter D Adams, Fabrizio d'Adda di Fagagna, Darren J Baker, Cleo L Bishop, Tamir Chandra, Manuel Collado, Jesus Gil, Vassilis Gorgoulis, Florian Gruber, Eiji Hara, Pidder Jansen-Dürr, Diana Jurk, Sundeep Khosla, James L Kirkland, Valery Krizhanovsky, Tohru Minamino, Laura J Niedernhofer, João F Passos, Nadja A R Ring, Heinz Redl, Paul D Robbins, Francis Rodier, Karin Scharffetter-Kochanek, John M Sedivy, Ewa Sikora, Kenneth Witwer, Thomas von Zglinicki, Maximina H Yun, Johannes Grillari, Marco Demaria
来源：
CELL
摘要：
细胞衰老是响应应激而触发的细胞命运，其特征是稳定的细胞周期停滞和分泌过多状态。它具有多种生物学作用，从组织修复到慢性疾病。研究体内衰老的新工具的开发为揭示其生理和病理作用以及测试衰老细胞作为治疗靶点铺平了道路。然而，缺乏特异性和广泛适用的标记物使得很难识别和表征组织和活体中的衰老细胞。为了解决这个问题，我们提供了名为“体内细胞衰老实验的最低信息”(MICSE) 的实用指南。它概述了啮齿动物组织、转基因模型、非哺乳动物系统、人体组织和肿瘤中的衰老标记物及其在衰老细胞识别和规范中的应用。这些指南提供了统一、最先进且易于使用的工具集，以增进我们对体内细胞衰老的理解。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。
Cellular senescence is a cell fate triggered in response to stress and is characterized by stable cell-cycle arrest and a hypersecretory state. It has diverse biological roles, ranging from tissue repair to chronic disease. The development of new tools to study senescence in vivo has paved the way for uncovering its physiological and pathological roles and testing senescent cells as a therapeutic target. However, the lack of specific and broadly applicable markers makes it difficult to identify and characterize senescent cells in tissues and living organisms. To address this, we provide practical guidelines called "minimum information for cellular senescence experimentation in vivo" (MICSE). It presents an overview of senescence markers in rodent tissues, transgenic models, non-mammalian systems, human tissues, and tumors and their use in the identification and specification of senescent cells. These guidelines provide a uniform, state-of-the-art, and accessible toolset to improve our understanding of cellular senescence in vivo.Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.