青年Vapers中的表观基因组失调：对疾病风险评估的影响
Epigenomic Dysregulation in Youth Vapers: Implications for Disease Risk Assessment
影响因子:5.30000
分区:医学2区 / 生化与分子生物学2区 细胞生物学2区 呼吸系统2区
发表日期:2025 Feb
作者:
Stella Tommasi, Luciano Brocchieri, Silvia Tornaletti, Ahmad Besaratinia
摘要
尽管持续的年轻人流行，但电子烟的长期健康后果在很大程度上尚不清楚。我们报告了与非用户相对于健康的年轻vaper和吸烟者口服细胞甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基甲基抗体的影响。 vaper和吸烟者在差异甲基化区域（DMR）的数量（831 vs. 2,863）方面有所不同，但它们在DNA甲基化，染色质状态，转录因子结合基序和途径的分布和模式上具有惊人的相似性。 VAPER和吸烟者之间与DMR相关的基因存在很大的重叠，其共享基因富含转录调控，信号传导，烟草使用障碍和与癌症相关途径的共享子集。显着性是在HIC1的启动子（癌症1中的高甲基化）中鉴定出常见的高甲基化DMR，这是肿瘤抑制基因在吸烟相关的癌症中经常沉默的基因。我们的数据支持青年vaper中的表观基因组失调与疾病风险之间的潜在联系。这些新颖的发现对公共卫生和烟草产品调节具有重要意义。
Abstract
Despite the ongoing epidemic of youth vaping, the long-term health consequences of electronic cigarette use are largely unknown. We report the effects of vaping versus smoking on the oral cell methylome of healthy young vapers and smokers relative to nonusers. Whereas vapers and smokers differ in the number of differentially methylated regions (DMRs) (831 vs. 2,863), they share striking similarities in the distribution and patterns of DNA methylation, chromatin states, transcription factor binding motifs, and pathways. There is substantial overlap in DMR-associated genes between vapers and smokers, with the shared subset of genes enriched for transcriptional regulation, signaling, tobacco use disorders, and cancer-related pathways. Of significance is the identification of a common hypermethylated DMR at the promoter of HIC1 (hypermethylated in cancer 1), a tumor suppressor gene frequently silenced in smoking-related cancers. Our data support a potential link between epigenomic dysregulation in youth vapers and disease risk. These novel findings have significant implications for public health and tobacco product regulation.