FCN1 基因编码 ficolin-1 蛋白，与多种疾病的发病机制有关，但其在肿瘤发生中的确切作用仍不清楚。本研究旨在阐明 FCN1 在不同癌症类型中的预后意义、免疫特征和治疗反应。利用多组学数据，我们进行了全面评估，包括组织特异性和单细胞特异性表达差异、泛癌症表达模式、影响 FCN1 表达的表观遗传修饰以及免疫微环境。我们的研究主要集中在 FCN1 和急性髓系白血病 (AML) 的临床预后属性、免疫特征、潜在分子机制和候选治疗药物上。此外，还进行了体外实验，以检查 FCN1 敲低对 AML 细胞系 U937 和 NB4 内细胞增殖、凋亡和细胞周期动态的影响。FCN1 表达在各种癌症中表现出广泛的失调。通过单变量和多变量 Cox 回归分析，FCN1 已被确定为 AML 的独立预后指标。免疫学研究阐明了 FCN1 参与调节肿瘤微环境中的炎症反应及其与治疗效果的相关性。值得注意的是，FCN1 的缺失会影响 U937 细胞和 NB4 细胞的增殖、凋亡和细胞周期动力学。这些发现强调 FCN1 作为一种有前景的泛癌生物标志物，指示巨噬细胞浸润，与肿瘤微环境和治疗反应密切相关，并且对于 AML 细胞系内的细胞机制至关重要。版权所有 © 2024zhong、song、li、liu、liu、guo 和 Liu。

The FCN1 gene encodes the ficolin-1 protein, implicated in the pathogenesis of various diseases, though its precise role in tumorigenesis remains elusive. This study aims to elucidate the prognostic significance, immune signature, and treatment response associated with FCN1 across diverse cancer types.Employing multi-omics data, we conducted a comprehensive assessment, encompassing tissue-specific and single-cell-specific expression disparities, pan-cancer expression patterns, epigenetic modifications affecting FCN1 expression, and the immune microenvironment. Our investigation primarily focused on the clinical prognostic attributes, immune profiles, potential molecular mechanisms, and candidate therapeutic agents concerning FCN1 and acute myeloid leukemia (AML). Additionally, in vitro experiments were performed to scrutinize the impact of FCN1 knockdown on cell proliferation, apoptosis, and cell cycle dynamics within the AML cell line U937 and NB4.FCN1 expression exhibits widespread dysregulation across various cancers. Through both univariate and multivariate Cox regression analyses, FCN1 has been identified as an independent prognostic indicator for AML. Immunological investigations elucidate FCN1's involvement in modulating inflammatory responses within the tumor microenvironment and its correlation with treatment efficacy. Remarkably, the deletion of FCN1 influences the proliferation, apoptosis, and cell cycle dynamics of U937 cells and NB4 cells.These findings underscore FCN1 as a promising pan-cancer biomarker indicative of macrophage infiltration, intimately linked with the tumor microenvironment and treatment responsiveness, and pivotal for cellular mechanisms within AML cell lines.Copyright © 2024 Zhong, Song, li, Liu, Liu, Guo and Liu.