独眼物种（蜜树）的抗癌潜力已在多种模型中得到证实。本研究调查了 C. subternata 和 C. genistoides 的水提取物和富含多酚 (PE) 的提取物以及芒果苷和橙皮苷对人肝脏 (HepG2) 和结肠 (HT-29) 中不同细胞生长参数的影响癌细胞。芒果苷和橙皮苷分别在C. genistoides 和C. subternata 中含量最高。独眼龙提取物具有最高的铁还原抗氧化能力。将细胞暴露于提取物和化合物后，测定细胞活力、增殖和死亡（细胞凋亡和自噬）。独眼巨人提取物降低了细胞活力并最大程度地抑制了细胞增殖，这与 ATP 的消耗有关。在 HepG2 细胞中，PE 提取物在降低细胞活力方面不如水提取物有效，但在抑制细胞增殖方面更有效。尽管破坏了细胞生长，但没有一种提取物诱导细胞凋亡。水提取物影响两种癌细胞的自噬。据推测，不同提取物可能通过多酚/铁相互作用破坏线粒体膜完整性；然而，芒果苷和橙皮苷没有任何作用，这表明其他多酚和/或化合物之间复杂的相互作用可能是提取物不同细胞毒性和/或细胞保护作用的原因。© 2024 作者。食品科学

The anti-cancer potential of Cyclopia species (honeybush) has been demonstrated in several models. The present study investigated the effects of aqueous and polyphenol-enriched (PE) extracts of C. subternata and C. genistoides, as well as mangiferin and hesperidin, on different cell growth parameters in human liver (HepG2) and colon (HT-29) cancer cells. Mangiferin and hesperidin were most abundant in C. genistoides and C. subternata, respectively. Cyclopia subternata extracts had the highest ferric-reducing antioxidant capacity. Following exposure of the cells to the extracts and compounds, cell viability, proliferation, and death (apoptosis and autophagy) were determined. Cyclopia subternata extracts reduced cell viability and inhibited cell proliferation the most, associated with depletion of ATP. In HepG2 cells, the PE extracts were less effective than the aqueous extracts in reducing cell viability but more effective in inhibiting cell proliferation. Despite disrupting cell growth, none of the extracts induced apoptosis. The aqueous extracts affected autophagy in both cancer cells. Disruption of mitochondrial membrane integrity by the different extracts, presumably via polyphenol/iron interactions, is postulated to be involved; however, mangiferin and hesperidin had no effect, suggesting that other polyphenols and/or complex interactions between compounds are likely responsible for the differential cytotoxic and/or cytoprotective effects of the extracts.© 2024 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.