IGFLR1是一种新型生物标志物，一些证据表明它参与结直肠癌的免疫微环境。在此，我们探讨IGFLR1在结直肠癌中的表达及其与预后以及免疫细胞浸润的关系，旨在为IGFLR1的进一步研究提供基础。在符合条件的组织中进行 IGFLR1、TIM-3、FOXP3、CD4、CD8 和 PD-1 的免疫组织化学染色，以分析 IGFLR1 的表达及其与预后和免疫细胞浸润的关系。然后，我们从 TCGA 中筛选结肠癌样本，并根据 IGFLR1 相关基因对患者进行分组。我们还评估了 IGFLR1 的共表达和免疫相关通路，以确定其在结直肠癌中的潜在机制。当P<0.05时，结果被认为具有统计学意义。 IGFLR1及IGFLR1相关基因与预后和免疫细胞浸润相关（P < 0.05）。在II期和III期CRC组织和正常组织中，我们发现（1）IGFLR1在细胞膜和细胞质中均表达，并且在癌组织和正常组织之间存在差异表达。 IGFLR1 表达与 FOXP3、CD8 和性别相关，但与微卫星不稳定性无关。 (2)IGFLR1是独立的预后因素，IGFLR1高的患者预后较好。 (3)包含IGFLR1、FOXP3、PD-1和CD4的模型显示出良好的预后分层能力。 (4)IGFLR1与GATA3之间存在显着的相互作用，并且IGFLR1与INFR通路中的相关因子存在显着的共表达。 IGFLR1 已成为与 CRC 患者疾病预后和免疫细胞浸润相关的新分子，并显示出良好的预测患者预后的能力。© 2024。作者。

IGFLR1 is a novel biomarker, and some evidences suggested that is involved in the immune microenvironment of CRC. Here, we explored the expression of IGFLR1 and its association with the prognosis as well as immune cell infiltration in CRC, with the aim to provide a basis for further studies on IGFLR1. Immunohistochemical staining for IGFLR1, TIM-3, FOXP3, CD4, CD8, and PD-1 was performed in eligible tissues to analyze the expression of IGFLR1 and its association with prognosis and immune cell infiltration. Then, we screened colon cancer samples from TCGA and grouped patients according to IGFLR1-related genes. We also evaluated the co-expression and immune-related pathways of IGFLR1 to identify the potential mechanism of it in CRC. When P < 0.05, the results were considered statistically significant. IGFLR1 and IGFLR1-related genes were associated with the prognosis and immune cell infiltration (P < 0.05). In stage II and III CRC tissue and normal tissue, we found (1) IGFLR1 was expressed in both the cell membrane and cytoplasm and which was differentially expressed between cancer tissue and normal tissue. IGFLR1 expression was associated with the expression of FOXP3, CD8, and gender but was not associated with microsatellite instability. (2) IGFLR1 was an independent prognostic factor and patients with high IGFLR1 had a better prognosis. (3) A model including IGFLR1, FOXP3, PD-1, and CD4 showed good prognostic stratification ability. (4) There was a significant interaction between IGFLR1 and GATA3, and IGFLR1 had a significant co-expression with related factors in the INFR pathway. IGFLR1 has emerged as a new molecule related to disease prognosis and immune cell infiltration in CRC patients and showed a good ability to predict the prognosis of patients.© 2024. The Author(s).