实体瘤内的缺氧环境限制了光动力疗法的有效性。在这里，我们演示了使用产氧纳米酶（CeO2、Fe3O4 和 MnO2）来改善光动力效应。使用 Box Behnken 实验设计获得了辐照工艺参数的优化组合。研究了吲哚菁绿、IR 820 及其与氧气发生器的不同组合对口腔癌的影响。动态光散射技术显示CeO2、MnO2和Fe3O4的平均粒径分别为211±16、157±28、143±19nm，PDI分别为0.23、0.28和0.20，zeta电位为-2.6±0.45，-分别为 2.4 ± 0.60 和 -6.1 ± 0.23 mV。使用紫外-可见光、FTIR 和 X 射线光子光谱证实了金属氧化物的形成。 CeO2、MnO2 和 Fe3O4 在 H2O2 存在下 2 分钟内产生的溶解氧量分别为 1.7 ± 0.15、1.7 ± 0.16 和 1.4 ± 0.12 mg/l。 FaDu 口腔癌球体模型中的生长抑制研究表明，照射后 ICG 和 IR 820 的生长减少显着 (P < 0.05)，分别从 81 ± 2.9 和 88 ± 2.1% 增加到 97 ± 1.2 和 99 ± 1.0% （808 nm 激光，1 W/cm2，5 分钟）在 CeO2 (25 µg/ml) 存在下。总之，产氧纳米酶可以改善 ICG 和 IR 820 的光动力效应。版权所有 © 2024 Elsevier B.V. 保留所有权利。

The hypoxic environment within a solid tumor is a limitation to the effectiveness of photodynamic therapy. Here, we demonstrate the use of oxygen generating nanozymes (CeO2, Fe3O4, and MnO2) to improve the photodynamic effect. The optimized combination of process parameters for irradiation was obtained using the Box Behnken experimental design. Indocyanine green, IR 820, and their different combinations with oxygen generators were studied for their effect on oral carcinoma. Dynamic light scattering technique showed the average particle size of CeO2, MnO2, and Fe3O4 to be 211 ± 16, and 157 ± 28, 143 ± 19 nm with PDI of 0.23, 0.28 and 0.20 and a zeta potential of -2.6 ± 0.45, -2.4 ± 0.60 and  -6.1 ± 0.23 mV, respectively. The formation of metal oxides was confirmed using UV-visible, FTIR, and X-ray photon spectroscopies. The amount of dissolved oxygen produced by CeO2, MnO2, and Fe3O4 in the presence of H2O2 within 2 min was 1.7 ± 0.15, 1.7 ± 0.16, and 1.4 ± 0.12 mg/l, respectively. Growth inhibition studies in the FaDu oral carcinoma spheroid model showed a significant (P < 0.05) increase in growth reduction from 81 ± 2.9 and 88 ± 2.1% to 97 ± 1.2 and 99 ± 1.0% for ICG and IR 820, respectively, after irradiation (808 nm laser, 1 W/cm2, 5 min) in the presence of CeO2 (25 μg/ml). In conclusion, oxygen-generating nanozymes can improve the photodynamic effect of ICG and IR 820.Copyright © 2024 Elsevier B.V. All rights reserved.