海藻酸盐是从褐藻中获得的天然多糖，具有有益的健康功效，广泛应用于营养保健品领域和制药行业。本研究致力于优化从不同褐海藻中提取海藻酸盐。采用 Box-Behnken 设计 (BBD)，通过分析温度（30、40 和 50°C）、时间（60、120 和 180 分钟）和碱浓度的影响，优化从海藻中提取海藻酸盐。 （1%、2% 和 3%）对提取率和糖醛酸含量的影响。记录的使海藻酸盐产量及其糖醛酸含量最大化的最佳条件是碱浓度为2.5%，温度为39.95°C，持续102.5分钟。使用 BBD 获得的优化参数来比较从 P. pavonica、马尾藻、Turbinaria turbinata 和 Dictyota dichotoma 中提取的藻酸盐。使用 FTIR、1H NMR 和 HPLC 来表征提取的藻酸盐。测试了海藻酸盐对抗自由基、乳腺癌细胞 (MCF-7)、某些病原微生物和 SARS-CoV-2 病毒的生物活性。在优化条件下，孔雀草中海藻酸盐的提取率为21.13±2.47% DW，灰灰藻中海藻酸盐的提取率为24.08±0.33% DW g/L，鼻甲藻中海藻酸盐的提取率为17.47±0.26% DW，芒萁的提取率为17.47±0.26% DW。比率为 19.57±3.60% DW。从 D. dichotoma 中提取的藻酸盐显示出最高的抗氧化、抗癌和抗病毒活性。版权所有 © 2024。由 Elsevier B.V. 出版。

Alginate is a natural polysaccharide obtained from brown seaweeds and having advantageous health usefulness, was employed extensively in nutraceutical sectors and the pharmaceutical industry. This research was devoted for optimization of alginate extraction from different brown seaweeds. A Box-Behnken Design (BBD) was used for the optimization of alginate extraction from Padina pavonica by analyzing the influence of temperature (30, 40, and 50 °C), time (60, 120, and 180 min), and alkaline concentration (1 %, 2 %, and 3 %) on extraction yield and uronic acid content. The optimal conditions recorded to maximize the alginate yield and its uronic content were an alkali concentration of 2.5 % and a temperature of 39.95 °C for 102.5 min. The optimized parameters achieved from BBD were used to compare alginate extraction from P. pavonica, Sargassum cinereum, Turbinaria turbinata, and Dictyota dichotoma. FTIR, 1H NMR, and HPLC were used to characterize the extracted alginate. The bioactivity of alginate against free radicals, breast cancer cells (MCF-7), some pathogenic microbes, and SARS-CoV-2 viruses was tested. Under the optimized conditions, alginate was extracted from P. pavonica at a rate of 21.13 ± 2.47 % DW, S. cinereum at 24.08 ± 0.33 % DW g/L, T. turbinata at 17.47 ± 0.26 % DW, and D. dichotoma at a rate of 19.57 ± 3.60 % DW. The alginate extracted from D. dichotoma showed the highest antioxidant, anticancer, and antiviral activity.Copyright © 2024. Published by Elsevier B.V.