免疫疗法已成为某些具有特定细胞和分子组成的癌症患者的新护理标准。然而，对于能够以高通量和患者特异性方式轻松评估免疫治疗效果的离体模型的需求仍然未得到满足。为了解决这个问题，我们开发了一种源自患者的肿瘤样微阵列系统，该系统具有重建的免疫微环境，该系统针对肿瘤浸润淋巴细胞功能的高内涵评估进行了优化。在这里，我们表明该系统为评估肿瘤免疫原性、表征对免疫调节剂的反应以及探索个性化免疫肿瘤学的新方法提供了前所未有的机会。© 2024。作者。

Immunotherapy has emerged as a new standard of care for certain cancer patients with specific cellular and molecular makeups. However, there is still an unmet need for ex vivo models able to readily assess the effectiveness of immunotherapeutic treatments in a high-throughput and patient-specific manner. To address this issue, we have developed a microarrayed system of patient-derived tumoroids with recreated immune microenvironments that are optimized for the high-content evaluation of tumor-infiltrating lymphocyte functionality. Here we show that this system offers unprecedented opportunities to evaluate tumor immunogenicity, characterize the response to immunomodulators, and explore novel approaches for personalized immuno-oncology.© 2024. The Author(s).