Gamma-H2AX 免疫荧光检测作为 DNA 双链断裂标记物已广受欢迎。在这项工作中，我们研究了 γ H2AX 免疫荧光测定作为生物剂量计在我们机构中估计剂量的潜在用途。选择了 7 名健康个体进行研究，并对前 5 名个体采集的血液样本进行低剂量照射远程钴装置中的 (0-10 cGy) 和高剂量 (50-500 cGy)。所有样品均进行 γ-H2AX 免疫荧光测定，并确定低剂量和高剂量的剂量反应校准曲线。为了验证确定的剂量-反应校准曲线，对从第六和第七个受试者获得的血液样本施加7.5cGy和250cGy的测试剂量。此外，还报告了完成测定所需的时间和成本。低剂量反应校准曲线和高剂量反应校准曲线的拟合优度 (R2) 值分别为 0.9829 和 0.9766。进行 γ-H2AX 免疫荧光测定所需的时间为 7 小时 30 分钟，每个样品的估计成本为 5000 卢比（约 60 美元）。根据这项研究，我们得出结论，确定的个体剂量反应校准曲线使用 γ-H2AX 免疫荧光测定法检测低剂量和高剂量范围的 γ 辐射，可用于生物剂量测定。此外，对于拥有现有共焦显微镜设施的机构来说，gamma-H2AX 免疫荧光测定可用作一种快速且经济高效的生物剂量测定工具。© 2024 大波兰癌症中心。

Gamma-H2AX immunofluorescence assay has gained popularity as a DNA double strand break marker. In this work, we have investigated the potential use of gamma H2AX immunofluorescence assay as a biological dosimeter for estimation of dose in our institution.Seven healthy individuals were selected for the study and the blood samples collected from the first five individuals were irradiated to low doses (0-10 cGy) and high doses (50-500 cGy) in a telecobalt unit. All the samples were processed for gamma-H2AX immunofluorescence assay and the dose-response calibration curves for low and high doses were determined. In order to validate the determined dose-response calibration curves, the blood samples obtained from the sixth and seventh subjects were delivered a test dose of 7.5 cGy and 250 cGy. In addition, time and cost required to complete the assay were also reported.The goodness of fit (R2) values was found to be 0.9829 and 0.9766 for low and high dose-response calibration curves. The time required to perform the gamma-H2AX immunofluorescence assay was found to be 7 hours and 30 minutes and the estimated cost per sample was 5000 rupees (~ 60 USD).Based on this study we conclude that the individual dose-response calibration curves determined with gamma-H2AX immunofluorescence assay for both low and high dose ranges of gamma radiation can be used for biological dosimetry. Further, the gamma-H2AX immunofluorescence assay can be used as a rapid cost-effective biodosimetric tool for institutions with an existing confocal microscope facility.© 2024 Greater Poland Cancer Centre.