乳腺癌是全球女性中最常见的恶性肿瘤之一，且发病率不断上升。体力活动，尤其是运动，已成为癌症预后的潜在重要调节因素，影响肿瘤生物学和患者预后。利用小鼠乳腺癌模型，我们建立了对照组和运动组，后者接受 21 天的自愿运动跑步。我们进行了RNA测序、生物信息学分析、泛癌分析和细胞实验来验证潜在机制。我们观察到运动显着减小了肿瘤的大小和重量，而体重没有显着变化，这表明体力活动可以调节肿瘤动态。运动后的 mRNA 测序显示，运动组中 CD300E 大幅下调，并伴有关键通路（例如癌症中的 MicroRNA 和钙信号通路）的改变。将我们的分析扩展到更广泛的癌症谱，CD300E 在多种癌症类型中表现出显着的表达变异性，在骨髓瘤、卵巢癌、肺癌和结直肠癌中表达显着上调。这种上调与较差的预后结果相关，强调了 CD300E 作为预后标志物和治疗靶点的潜在作用。此外，CD300E 表达与癌细胞增殖和凋亡相关。该研究强调了运动在调节与肿瘤生长相关的基因表达中的双重作用，以及 CD300E 作为癌症治疗靶点的潜力。鼓励进一步研究探索运动和 CD300E 影响癌症进展的机制，并制定可以提高临床环境中患者治疗效果的针对性策略。版权所有 © 2024 Luo、Zhu、Xu、Wan、He、Chen、Wang、Chen 和 Chen 。

Breast cancer ranks as one of the most prevalent malignancies among women globally, with increasing incidence rates. Physical activity, particularly exercise, has emerged as a potentially significant modifier of cancer prognosis, influencing tumor biology and patient outcomes.Using a murine breast cancer model, we established a control and an exercise group, where the latter was subjected to 21 days of voluntary running. RNA Sequencing, bioinformatics analysis, pan-cancer analysis, and cell experiments were performed to validate the underlying mechanisms.We observed that exercise significantly reduced tumor size and weight, without notable changes in body weight, suggesting that physical activity can modulate tumor dynamics. mRNA sequencing post-exercise revealed substantial downregulation of CD300E in the exercise group, accompanied by alterations in critical pathways such as MicroRNAs in cancers and the Calcium signaling pathway. Expanding our analysis to a broader cancer spectrum, CD300E demonstrated significant expression variability across multiple cancer types, with pronounced upregulation in myeloma, ovarian, lung, and colorectal cancers. This upregulation was correlated with poorer prognostic outcomes, emphasizing CD300E's potential role as a prognostic marker and therapeutic target. Moreover, CD300E expression was associated with cancer cell proliferation and apoptosis.The study highlights the dual role of exercise in modulating gene expression relevant to tumor growth and the potential of CD300E as a target in cancer therapeutics. Further research is encouraged to explore the mechanisms by which exercise and CD300E influence cancer progression and to develop targeted strategies that could enhance patient outcomes in clinical settings.Copyright © 2024 Luo, Zhu, Xu, Wan, He, Chen, Wang, Chen and Chen.