前列腺癌是男性第二常见的癌症死亡类型。本研究将泊洛沙姆涂层钴铁氧体的新型热疗应用作为在射频磁场 (RF-MF) 下热消灭 DU-145 人类前列腺癌细胞的新方法。采用水热法合成钴铁氧体纳米颗粒。然后，对纳米颗粒的结构、尺寸和形貌进行了表征。单独或结合集落形成方法和 MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑]研究合成纳米颗粒和 RF-MF 暴露对 DU-145 前列腺癌细胞的细胞毒性溴化物]测定。透射电子显微镜（TEM）证实纳米粒子的球形形态，尺寸为5.5±2.6nm。在RF-MF下用纳米颗粒处理的细胞的温度在15分钟后达到42.73±0.2℃。 RF-MF 处理或纳米颗粒并未显着影响细胞活力。然而，它们的组合消除了 53%±4% 的癌细胞。使用特定浓度的钴铁氧体纳米颗粒对人前列腺癌细胞 (DU-145) 进行体外热疗，根据集落形成测定结果显示，与单独用纳米颗粒或 RF-MF 处理的细胞相比，存活率降低。版权所有 © 2024 Nazanin Oroskhani 等人。

Prostate cancer is the second most frequent type of cancer death in men. This study refers to the novel hyperthermia application of poloxamer-coated cobalt ferrite as a new approach for thermal eradication of DU-145 human prostate cancerous cells under a radio frequency magnetic field (RF-MF). The hydrothermal method was applied for the synthesis of cobalt ferrite nanoparticles. Then, the structure, size, and morphology of nanoparticle were characterized. The cytotoxicity of the synthesized nanoparticles and RF-MF exposure on DU-145 prostate cancer cells was investigated separately or in combination with colony formation methods and MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. Transmission electron microscopy (TEM) confirmed the spherical morphology of nanoparticles with a size of 5.5 ± 2.6 nm. The temperature of cells treated with nanoparticles under RF-MF reached 42.73 ± 0.2°C after 15 min. RF-MF treatment or nanoparticles have not affected cell viability significantly. However, the combination of them eradicated 53% ± 4% of cancerous cells. In-vitro hyperthermia was performed on human prostate cancer cells (DU-145) with cobalt ferrite nanoparticles at specific concentrations that demonstrated a decrease in survival fraction based on colony formation assay compared to cells that were treated alone with nanoparticles or with RF-MF.Copyright © 2024 Nazanin Oroskhani et al.