本研究旨在评估生长素释放肽对脓毒症小鼠的心脏保护作用，重点关注其抗炎和抗氧化特性。 35 只雄性瑞士小鼠（8-12 周龄，23-33g）被随机分配到五组（每组 n = 7）：（1）正常，喂食常规饮食，（2）假手术，接受麻醉和剖腹手术， (3)脓毒症，进行盲肠结扎和穿刺，(4)媒介物，在盲肠结扎和穿刺后立即给予等体积的腹腔盐水注射，以及(5)经Ghrelin处理的，在紧随其后腹腔注射80μg/kg ghrelin盲肠结扎和穿刺。测量肿瘤坏死因子-α (TNF-α)、巨噬细胞迁移抑制因子 (MIF)、Toll 样受体 4 (TLR4) 和 8-epi-前列腺素 F2 α (8-epi-PGF2α) 的血清水平。还通过组织学评估了心脏损伤的程度。脓毒症组和赋形剂组的平均血清 TNF-α、MIF、TLR4 和 8-epi-PGF2α 水平显着高于正常组和假手术组。生长素释放肽治疗组的水平显着低于媒介物组和脓毒症组。组织学分析显示正常组和假手术组的心肌结构正常，而脓毒症组和媒介物组有严重的心肌损伤。生长素释放肽治疗组表现出与假手术组相似的组织学特征，表明心肌损伤减轻。胃饥饿素通过表现出强大的抗炎和抗氧化作用，改善小鼠脓毒症引起的心脏毒性。这些发现表明，生长素释放肽可能是预防脓毒症引起的心脏毒性的一种有前途的治疗候选药物。© 2024 by 作者。

This study aimed to evaluate the cardioprotective effects of ghrelin in septic mice, focusing on its anti-inflammatory and antioxidant properties. Thirty-five male Swiss mice (8-12 weeks old, 23-33g) were randomly assigned to five groups (n = 7 each): (1) Normal, fed usual diets, (2) Sham, subjected to anesthesia and laparotomy, (3) Sepsis, subjected to cecal ligation and puncture, (4) Vehicle, given an equivalent volume of intraperitoneal saline injections immediately after cecal ligation and puncture, and (5) Ghrelin-treated, administered 80 µg/kg ghrelin intraperitoneal injections immediately following cecal ligation and puncture. Serum levels of tumor necrosis factor-alpha (TNF-α), macrophage migration inhibitory factor (MIF), toll-like receptor 4 (TLR4), and 8-epi-prostaglandin F2 alpha (8-epi-PGF2α) were measured. The extent of cardiac damage was also evaluated histologically. The mean serum levels of TNF-α, MIF, TLR4, and 8-epi-PGF2α levels were significantly higher in the sepsis and vehicle groups than in the normal and sham groups. The levels were significantly lower in the ghrelin-treated group than in the vehicle and sepsis groups. Histological analysis revealed normal myocardial architecture in the normal and sham groups, whereas the sepsis and vehicle groups had severe myocardial injury. The ghrelin-treated group displayed histological features similar to the sham group, indicating reduced myocardial damage. Ghrelin ameliorated sepsis-induced cardiotoxicity in mice by exhibiting strong anti-inflammatory and antioxidant effects. These findings suggest that ghrelin may be a promising therapeutic candidate for the prevention of sepsis-induced cardiotoxicity.© 2024 by the authors.