膀胱癌（BC）是世界上第六大常见癌症，也是男性癌症死亡的第九大原因。既往研究表明肿瘤缺氧在BC的发生、发展中发挥着重要作用，但肿瘤缺氧在BC的预后和免疫浸润中的作用尚不清楚。我们的目的是进行生物信息学分析与临床分析相结合，以探讨缺氧在 BC 中的作用。我们从基因表达综合库 (GEO) 获取了包含 BC 队列中 mRNA 表达信息的数据集（GSE13507、GSE5287 和 GSE1827）并测量了使用基因集变异分析 (GSVA) 计算缺氧评分。然后采用X-tile法、log-rank检验和Pearson相关性检验分析缺氧评分与BC临床病理和免疫学特征之间的关系，并采用逐步Cox回归分析建立预后模型。与我们研究中BC的肿瘤分级、病理类型、侵袭和预后相关。此外，我们通过相关性分析确定缺氧与多种免疫细胞的浸润丰度密切相关，并进一步发现肿瘤免疫细胞浸润与BC的肿瘤分级和肿瘤类型显着相关。此外，我们基于缺氧评分和肿瘤免疫浸润构建了多个模型，C指数范围为0.703和0.888，在预测BC预后方面表现出良好的性能。我们的研究表明，缺氧在疾病进展、预后中起着重要作用。 ，以及BC的肿瘤免疫浸润。我们基于缺氧和肿瘤免疫浸润的模型对 BC 患者的预后和治疗起到指导作用。2024 转化癌症研究。版权所有。

Bladder cancer (BC) is the sixth most common cancer and the ninth leading cause of cancer death among men in the world. Previous studies have shown that tumor hypoxia plays an important role in the occurrence and development of BC, but the role of tumor hypoxia in the prognosis and immune infiltration of BC remains unclear. Our aim was to perform a bioinformatics analysis combined with a clinical analysis to explore the roles of hypoxia in BC.We acquired datasets (GSE13507, GSE5287, and GSE1827) containing mRNA expression information from BC cohorts from the Gene Expression Omnibus (GEO) and measured the Hypoxia score using the Gene Set Variation Analysis (GSVA). Then we used X-tile method and log-rank test and Pearson's correlation test to analyze the relation among the Hypoxia score and the clinicopathological and immunological characteristics of BC and used stepwise Cox regression analysis to establish a Prognostic model.Hypoxia was found to be closely associated with tumor grade, pathological type, invasion, and prognosis of BC in our study. Moreover, we determined that hypoxia was closely related to the infiltration abundance of multiple immune cells through a correlation analysis, and the tumor immune cell infiltration was further found to be significantly associated with the tumor grade and tumor type of BC. Furthermore, we constructed several models based on the Hypoxia score and tumor immune infiltration with C-indexes ranging from 0.703 and 0.888, which showed good performance in predicting the prognosis of BC.Our study showed that hypoxia plays an important role in the progression, prognosis, and tumor immune infiltration of BC. Our models based on hypoxia and tumor immune infiltration play a guiding role in the prognosis and treatment of BC patients.2024 Translational Cancer Research. All rights reserved.