内质网应激（ERS）可能是治疗恶性肿瘤的一种策略。此外，长链非编码RNA（lncRNA）可以促进肿瘤的发生和进展，并预测癌症的预后。然而，ERS相关lncRNA在肺腺癌（LUAD）中的预后价值尚未见报道。与LUAD相关的信使RNA（mRNA）、microRNA（miRNA）和lncRNA表达数据均在公共数据库（TCGA和GEO数据库）中获得。获得了预后ERS相关的差异表达lncRNA（ERS-DEL），并通过Cox回归分析用于构建ERS相关模型。此外，我们进一步筛选了独立的预后因素并构建了列线图。此外，还进行了基因的富集分析以研究其功能。建立lncRNA-miRNA-mRNA网络来探索lncRNA的机制。最后，利用qRT-PCR检测lncRNA的表达水平。鉴定出30个ERS-DEL，并基于AF131215.2、LINC00472、LINC01352、RP1-78O14.1、RP11-253E3构建ERS相关特征。 3、RP11-98D18.9 和 SNHG12。基因集富集分析表明，高风险组中的基因主要集中于mRNA结合的调节，而低风险组中的基因则显着集中于纤毛的蛋白质定位。还建立了包含 7 个特征 lncRNA、23 个 miRNA 和 128 个 mRNA 的 lncRNA-miRNA-mRNA 网络。最终，通过实时定量聚合酶链反应证实7个预后lncRNA的表达与分析一致。构建了包含7个预后lncRNA的ERS相关特征，这为研究ERS相关lncRNA在癌症中的作用提供了新的思路。 LUAD.© 2024 约翰·威利

Endoplasmic reticulum stress (ERS) could be a strategy for treating malignant tumors. Moreover, long noncoding RNAs (lncRNAs) can promote tumorigenesis and progression, and forecast the prognosis of cancers. Nevertheless, the prognostic value of ERS-related lncRNAs has not been reported in lung adenocarcinoma (LUAD).The messenger RNA (mRNA), microRNA (miRNA) and lncRNA expression data related to LUAD were obtained in public databases (TCGA and GEO databases). Prognostic ERS-related differentially expressed lncRNAs (ERS-DELs) were obtained and used to build an ERS-related model by Cox regression analysis. Moreover, we further screened independent prognostic elements and built a nomogram. Furthermore, enrichment analysis of genes was conducted to investigate the functions. A lncRNA-miRNA-mRNA network was built to explore mechanism of lncRNAs. Finally, qRT-PCR was utilized to examine the expression levels of lncRNAs.30 ERS-DELs were identified, and an ERS-related signature was built based on AF131215.2, LINC00472, LINC01352, RP1-78O14.1, RP11-253E3.3, RP11-98D18.9, and SNHG12. Gene set enrichment analysis indicated that genes in the high-risk group were chiefly focused on the regulation of mRNA binding, and genes in the low-risk group were significantly focused on protein localization to cilia. A lncRNA-miRNA-mRNA network, containing 7 signature lncRNAs, 23 miRNAs, and 128 mRNAs, was also established. Eventually, quantitative real-time polymerase chain reaction was used to confirm that seven prognostic lncRNAs had a consistent expression with the analysis.An ERS-related signature containing seven prognostic lncRNAs was built, which offered new thinking concerning the role of ERS-related lncRNAs in LUAD.© 2024 John Wiley & Sons Ltd.