凭借高灵敏度、单碱基分辨率、多重检测能力和核酸识别可编程性等特性，基于 Argonaute (Ago) 的生物传感检测越来越被认为是精确识别和定量目标分析物最有前途的工具之一。 Agos 被用作这些强大诊断方法的高度特异性序列识别元件，正在彻底改变核酸靶标的检测方式。为了发挥基于 Ago 的生物传感检测的潜力，有必要对这一新兴且快速发展的技术进行系统、全面的总结。文章首先简要概述了Agos的结构和功能，然后回顾了利用Agos传感技术检测各种目标的最新进展，并对病毒、肿瘤生物标志物、病原体、支原体和寄生虫等进行综合分析。然后讨论了这些平台的重要性和好处。此外，作者还分享了对现有挑战的主观观点，并为 Agos 检测的未来进展提供了相关指导。最后，还对该领域基于Ago的传感技术的未来研究前景进行了展望。因此，这篇综述预计将为更广泛的研究人员群体提供有价值的信息和新鲜的观点。版权所有 © 2024。由 Elsevier B.V. 出版。

With the attributes of high sensitivity, single-base resolution, multiplex detection capability, and programmability upon nucleic acid recognition, Argonaute (Ago)-based biosensing assays are increasingly recognized as one of the most promising tools for precise identification and quantification of target analytes. Employed as highly specific sequence recognition elements of these robust diagnostic methods, Agos are revolutionizing how nucleic acid targets are detected. A systematic and comprehensive summary of this emerging and rapid-advancing technology is necessary to give play to the potential of Ago-based biosensing assays. The structure and function of Agos were briefly overviewed at the beginning of the work, followed by a review of the recent advancements in employing Agos sensing for detecting various targets with a comprehensive analysis such as viruses, tumor biomarkers, pathogens, mycoplasma, and parasite. The significance and benefits of these platforms were then deliberated. In addition, the authors shared subjective viewpoints on the existing challenges and offered relevant guidance for the future progress of Agos assays. Finally, the future research outlook regarding Ago-based sensing in this field was also outlined. As such, this review is expected to offer valuable information and fresh perspectives for a broader group of researchers.Copyright © 2024. Published by Elsevier B.V.