可编程且超高效的Argonaute蛋白介导核酸检测技术综述
Programmable and ultra-efficient Argonaute protein-mediated nucleic acid tests: A review
DOI 原文链接
用sci-hub下载
如无法下载，请从 Sci-Hub 选择可用站点尝试。
影响因子:8.5
分区:生物学2区 Top / 生化与分子生物学2区 应用化学2区 高分子科学2区
发表日期:2024 Oct
作者:
Jinlong Jiao, Dandan Zeng, Yafang Wu, Chentao Li, Tianlu Mo
DOI:
10.1016/j.ijbiomac.2024.134755
摘要
具有高灵敏度、单碱基分辨率、多重检测能力和可编程识别核酸特性的Argonaute（Ago）生物传感检测技术正日益成为精准识别和定量目标分析物的最有潜力的工具之一。作为这些稳健诊断技术的高度特异性序列识别元件，Ago正革新核酸目标的检测方式。本文系统综述了这一新兴且快速发展的技术的结构与功能，回顾了近年来在病毒、肿瘤标志物、病原体、支原体和寄生虫等多种目标检测中的应用进展，并分析了这些平台的意义与优势。同时，作者还就当前面临的挑战提出了主观观点，并为未来的研究方向提供了指导。最后，展望了Ago基检测技术在该领域的未来发展前景。
Abstract
With the attributes of high sensitivity, single-base resolution, multiplex detection capability, and programmability upon nucleic acid recognition, Argonaute (Ago)-based biosensing assays are increasingly recognized as one of the most promising tools for precise identification and quantification of target analytes. Employed as highly specific sequence recognition elements of these robust diagnostic methods, Agos are revolutionizing how nucleic acid targets are detected. A systematic and comprehensive summary of this emerging and rapid-advancing technology is necessary to give play to the potential of Ago-based biosensing assays. The structure and function of Agos were briefly overviewed at the beginning of the work, followed by a review of the recent advancements in employing Agos sensing for detecting various targets with a comprehensive analysis such as viruses, tumor biomarkers, pathogens, mycoplasma, and parasite. The significance and benefits of these platforms were then deliberated. In addition, the authors shared subjective viewpoints on the existing challenges and offered relevant guidance for the future progress of Agos assays. Finally, the future research outlook regarding Ago-based sensing in this field was also outlined. As such, this review is expected to offer valuable information and fresh perspectives for a broader group of researchers.