可编程和超高效的Argonaute蛋白质介导的核酸测试：综述
Programmable and ultra-efficient Argonaute protein-mediated nucleic acid tests: A review
影响因子:8.50000
分区:生物学2区 Top / 生化与分子生物学2区 应用化学2区 高分子科学2区
发表日期:2024 Oct
作者:
Jinlong Jiao, Dandan Zeng, Yafang Wu, Chentao Li, Tianlu Mo
摘要
具有高灵敏度，单基碱分辨率，多重检测能力和可编程性识别的属性，基于ARGONATE（ARGONAUTE（ARGO）基于ARGONATE（ARGONATE）的生物传感测定越来越被认为是用于精确识别和量化目标分析物的最有希望的工具之一。作为这些可靠诊断方法的高度特定序列识别元件，AGO正在革新检测到核酸靶标。对于这种新兴和快速放松技术的系统和全面的总结对于发挥基于AGO的生物传感测定的潜力是必要的。在工作开始时，对AGOS的结构和功能进行了简要概述，随后综述了使用AGOS传感的最新进步来检测各种靶标，例如病毒，肿瘤生物标志物，病原体，肉芽肿和寄生虫。然后审议了这些平台的重要性和好处。此外，作者在现有挑战上分享了主观的观点，并为AGOS分析的未来进展提供了相关指导。最后，还概述了有关该领域基于AGO的感知的未来研究前景。因此，预计该评论将为更广泛的研究人员提供有价值的信息和新观点。
Abstract
With the attributes of high sensitivity, single-base resolution, multiplex detection capability, and programmability upon nucleic acid recognition, Argonaute (Ago)-based biosensing assays are increasingly recognized as one of the most promising tools for precise identification and quantification of target analytes. Employed as highly specific sequence recognition elements of these robust diagnostic methods, Agos are revolutionizing how nucleic acid targets are detected. A systematic and comprehensive summary of this emerging and rapid-advancing technology is necessary to give play to the potential of Ago-based biosensing assays. The structure and function of Agos were briefly overviewed at the beginning of the work, followed by a review of the recent advancements in employing Agos sensing for detecting various targets with a comprehensive analysis such as viruses, tumor biomarkers, pathogens, mycoplasma, and parasite. The significance and benefits of these platforms were then deliberated. In addition, the authors shared subjective viewpoints on the existing challenges and offered relevant guidance for the future progress of Agos assays. Finally, the future research outlook regarding Ago-based sensing in this field was also outlined. As such, this review is expected to offer valuable information and fresh perspectives for a broader group of researchers.