商业种猪睾丸肿瘤的宏观、组织学及免疫组化研究
Testicular tumors in commercial boars with infertility: A gross, histologic, and immunohistochemical study
DOI 原文链接
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影响因子:1.7
分区:农林科学2区 / 兽医学2区 病理学3区
发表日期:2025 Jan
作者:
Raquel Ausejo, María Victoria Falceto, Olga Mitjana, Ana María Garrido, Juan José Badiola, Bernardino Moreno
DOI:
10.1177/03009858241269836
摘要
猪的肿瘤较少见，尤其是睾丸肿瘤。本研究描述了一系列商业种猪睾丸肿瘤的病理和免疫组化特征，涉及具有生育问题的19只猪（19/333，5.9%）。宏观观察表明，13只（13/19，68%）猪的睾丸中发现肿瘤，而6例（6/19，32%）仅通过显微镜检查发现。睾丸肿大在1只猪中观察到，其余多数在切除阴囊皮肤或切片后发现。组织学上，肿瘤被分类为精原细胞瘤（16/19，84%）、混合生殖细胞-间质肿瘤（2/19，11%）及B细胞淋巴瘤（1/19，5%）。精原细胞瘤呈三种生长模式：管内（6/16，38%）、弥漫（4/16，25%）和管内/弥漫（6/16，38%）。所有未在宏观上明显的肿瘤均为管内精原细胞瘤。观察到2例存在睾丸内转移，1例存在位于精索静脉丛的睾丸外转移。1例精原细胞瘤还涉及睾丸网。免疫组化显示，所有管内精原细胞瘤细胞对c-kit、细胞角蛋白和波形蛋白均为阴性。弥漫性精原细胞瘤中c-kit和细胞角蛋白亦为阴性，有些区域的波形蛋白表现出颗粒状或核周细胞质标记。PAX-5和CD-3抗体将淋巴瘤归类为B细胞淋巴瘤。本研究提示，种猪中的睾丸肿瘤可能比以前报道的更常见，尤其是在进行显微镜检查时，同时显示出猪的睾丸肿瘤主要为精原细胞瘤。
Abstract
Tumors in boars are uncommon, and testicular tumors even rarer. This study describes the pathological and immunohistochemical characteristics of a case series of testicular tumors in commercial boars with fertility problems. Tumors were detected in 19 of 333 animals (19/333, 5.9%). Macroscopically, tumors were observed in 13 (13/19, 68%) boars, while 6 cases (6/19, 32%) were only detected by microscopic examination. Testicular enlargement was observed in 1 boar, while in the others, tumors were only observed after removal of the scrotal skin or after sectioning of the testis. Histologically, tumors were classified as seminomas (16/19, 84%), mixed germ cell-stromal tumors (2/19, 11%), and B-cell lymphoma (1/19, 5%). Seminomas had 3 different growth patterns: intratubular (6/16, 38%), diffuse (4/16, 25%), and intratubular/diffuse (6/16, 38%). All tumors that were not evident on macroscopic examination were intratubular seminomas. Intratesticular metastases were observed in 2 cases and extratesticular metastases, located in the pampiniform plexus, were observed in 1 case. In 1 seminoma, the rete testis was also involved. By immunohistochemistry, all intratubular seminomas were negative for c-kit, cytokeratin, and vimentin. In diffuse seminomas, c-kit and cytokeratin were also negative, while vimentin showed granular or perinuclear cytoplasmic labeling in some areas. PAX-5 and CD-3 antibodies classified the lymphoma as a B-cell lymphoma. This study suggests that testicular tumors in boars may be more common than previously reported, especially when microscopic examination is performed. It also shows that testicular tumors in pigs are predominantly seminomas.