原发性开角型青光眼 (POAG) 根据眼压进行细分。正常眼压性青光眼 (NTG) 患者从未测量过高眼压 (IOP)，而高眼压 (OHT) 患者眼压较高，但没有青光眼迹象。尽管眼内压被认为是所有青光眼患者的危险因素，但可以合理地假设炎症等其他危险因素也发挥了作用。我们的目的是表征 NTG (n = 10)、OHT (n = 10) 和对照 (n = 10) 患者血浆缺氧期间的蛋白质组和细胞因子谱。参与者先处于缺氧状态 2 小时，然后再处于常氧状态 30 分钟。在缺氧之前（“基线”）、缺氧期间（“缺氧”）和缺氧之后（“恢复”）采集样本。进行了基于液相色谱与质谱联用 (LC-MS) 的蛋白质组学研究。通过 Luminex 测定法测量细胞因子。生物信息学分析表明补体和凝血级联参与 NTG 和 OHT。高密度脂蛋白 3 (HDL3) 载脂蛋白的调节表明胆固醇代谢的变化与 OHT 相关。与对照组相比，缺氧降低了 OHT 患者的肿瘤坏死因子-α (TNF-α) 水平。缺氧期间，与对照组相比，NTG 患者的白细胞介素-1β (IL-1β) 和 C 反应蛋白 (CRP) 循环水平降低。 NTG 和 OHT 患者康复后，血浆白细胞介素 6 (IL-6) 表达上调。目前的结果表明，NTG 和 OHT 患者的全身免疫反应增强，这与青光眼的致病事件相关。载脂蛋白可能具有抗炎作用，使 OHT 患者能够在高眼压的情况下抵抗炎症和青光眼的发展。© 2024。作者。

Primary open-angle glaucoma (POAG) is subdivided depending on eye pressure. Patients with normal-tension glaucoma (NTG) have never had high intraocular pressure (IOP) measured while patients with ocular hypertension (OHT) have high eye pressure but no signs of glaucoma. Although IOP is considered to be a risk factor for all glaucoma patients, it is reasonable to assume that other risk factors such as inflammation play a role. We aimed to characterize the proteome and cytokine profile during hypoxia in plasma from patients with NTG (n = 10), OHT (n = 10), and controls (n = 10). Participants were exposed to hypoxia for two hours, followed by 30 min of normoxia. Samples were taken before ("baseline"), during ("hypoxia"), and after hypoxia ("recovery"). Proteomics based on liquid chromatography coupled with mass spectrometry (LC-MS) was performed. Cytokines were measured by Luminex assays. Bioinformatic analyses indicated the involvement of complement and coagulation cascades in NTG and OHT. Regulation of high-density lipoprotein 3 (HDL3) apolipoproteins suggested that changes in cholesterol metabolism are related to OHT. Hypoxia decreased the level of tumor necrosis factor-α (TNF-α) in OHT patients compared to controls. Circulating levels of interleukin-1β (IL-1β) and C-reactive protein (CRP) were decreased in NTG patients compared to controls during hypoxia. After recovery, plasma interleukin-6 (IL-6) was upregulated in patients with NTG and OHT. Current results indicate an enhanced systemic immune response in patients with NTG and OHT, which correlates with pathogenic events in glaucoma. Apolipoproteins may have anti-inflammatory effects, enabling OHT patients to withstand inflammation and development of glaucoma despite high IOP.© 2024. The Author(s).